Mitochondrial DNA copy numbers in gastric cancer tissues: a possible biomarker for estimating cancer progression.

IF 1.9 4区 医学 Q3 ONCOLOGY
Hisanori Fukunaga, Mayuko Fukunaga
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引用次数: 0

Abstract

Background: Mitochondria have their own genome (mtDNA), which in humans is a circular multi-copy genome consisting of 16 569 base pairs. Abnormalities in the mtDNA have been reported to correlate with various age-related pathophysiologies.

Methods: Based on a total of 182 DNA samples extracted from gastric cancer tissues, we measured mtDNA copy numbers (mtDNA-CN) using real-time polymerase chain reaction (PCR) and then examined alongside sex, age, tumor stage, Laurén classification, and the overexpression of Human Epidermal Growth Factor Receptor 2 (HER2).

Results: We found no sex differences in mtDNA-CN and no correlation with age, but significant differences according to tumor stage. The mtDNAcn of intestinal type by Laurén classification was significantly larger than that of diffuse type. There was no significant difference in mtDNA-CN between HER2-positive and -negative tissues. Multiple regression analyses showed that only the tumor stage was a significant variable, while Laurén classification was not.

Conclusion: These results indicate that mitochondrial genomic abnormalities contribute the progression of gastric cancer independently of HER2 overexpression, and may shed light on the emerging role of mtDNA-CN in situ as a possible biomarker for estimating cancer progression.

胃癌组织中的线粒体DNA拷贝数:估计癌症进展的可能生物标志物。
背景:线粒体有自己的基因组(mtDNA),在人类中是一个由16569个碱基对组成的环状多拷贝基因组。据报道,mtDNA的异常与各种与年龄相关的病理生理有关。方法:采用实时聚合酶链式反应(real-time polymerase chain reaction, PCR)检测182例胃癌组织mtDNA拷贝数(mtDNA- cn),并与性别、年龄、肿瘤分期、laur分类、人表皮生长因子受体2 (HER2)过表达进行比较。结果:mtDNA-CN无性别差异,与年龄无关,但肿瘤分期差异显著。lauracn分类肠型mtDNAcn明显大于弥漫型。her2阳性组织与her2阴性组织mtDNA-CN差异无统计学意义。多元回归分析显示,只有肿瘤分期是显著变量,而laurassan分类无显著差异。结论:这些结果表明,线粒体基因组异常独立于HER2过表达促进胃癌的进展,并可能揭示mtDNA-CN原位作为估计癌症进展的可能生物标志物的新作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.70
自引率
8.30%
发文量
177
审稿时长
3-8 weeks
期刊介绍: Japanese Journal of Clinical Oncology is a multidisciplinary journal for clinical oncologists which strives to publish high quality manuscripts addressing medical oncology, clinical trials, radiology, surgery, basic research, and palliative care. The journal aims to contribute to the world"s scientific community with special attention to the area of clinical oncology and the Asian region. JJCO publishes various articles types including: ・Original Articles ・Case Reports ・Clinical Trial Notes ・Cancer Genetics Reports ・Epidemiology Notes ・Technical Notes ・Short Communications ・Letters to the Editors ・Solicited Reviews
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