Association of CDKAL1 gene polymorphisms variations with gestational diabetes mellitus risk in women: A case-control study and meta-analysis.

Abstract

Background: Gestational diabetes mellitus (GDM) has seen a significant rise and has become a growing concern worldwide, especially in Asian populations. Genetic factors, such as variations in the CDKAL1 gene, have been linked to its development. However, existing research on this connection is limited and inconclusive, highlighting the need for further investigation. This study aims to explore the association between CDKAL1 gene polymorphisms and GDM risk in a Chinese population using a comprehensive case-control study and meta-analysis.

Methods: The SNPscan™ genotyping assay was used to genotype rs7754840 and rs7756992, in 502 control participants and 500 GDM patients. ANOVA, T-test, chi-square test, logistic regression, and other statistical tests were used to determine the differences in genotypes and alleles and their associations to the risk of GDM. Additionally, a meta-analysis of existing studies on CDKAL1 polymorphisms and GDM was performed to provide a broader context and resolve inconsistencies in the literature.

Results: The GDM group had a significantly older average mean age and higher blood pressure, and fasting plasma glucose levels than the control group (P < 0.05). CDKAL1 rs7754840 showed significant associations under codominant homozygous model (CC vs. GG: OR = 1.748; 95% CI: 1.178-2.593; P = 0.006). After adjusting, these results indicated an association between CDKAL1 rs7754840 and increased risk of GDM in the codominant model (OR = 1.715; 95% CI: 1.133-2.595; P = 0.011). However, further analysis revealed no significant associations under all genetic models for CDKAL1 rs7756992. The study found that individuals under 30 with the rs7754840 CC genotype had higher fasting glucose and postprandial glucose levels (P < 0.05) compared to those with the GG genotype. Figure 3 A demonstrated a modest association between the CDKAL1 and GDM susceptibility (OR 1.16, 95% CI 1.104-1.29, P = 0.0258).

Conclusion: Individuals with the CDKAL1 rs7754840 polymorphism was associated to an increased risk of GDM, whereas rs7756992 did not show significant association with GDM risk. These results provide a theoretical foundation for GDM testing to mitigate its associated complications by enhancing our ability to predict, prevent and manage GDM. Ultimately improving outcomes for both mothers and their children. This research contributes to the growing evidence of genetic predisposition to GDM and highlights the importance of CDKAL1 as a potential genetic marker for GDM risk assessment.