Investigation of a point-of-care viscoelastic coagulation monitor and its comparison to thromboelastography in 9 female southern white rhinoceros (Ceratotherium simum simum).

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

American journal of veterinary research Pub Date : 2025-04-17 DOI:10.2460/ajvr.24.10.0322

Ashlyn C Heniff, Alex M Lynch, Laura K Ruterbories, Larry J Minter, Timothy A Georoff, Julie A Balko

{"title":"Investigation of a point-of-care viscoelastic coagulation monitor and its comparison to thromboelastography in 9 female southern white rhinoceros (Ceratotherium simum simum).","authors":"Ashlyn C Heniff, Alex M Lynch, Laura K Ruterbories, Larry J Minter, Timothy A Georoff, Julie A Balko","doi":"10.2460/ajvr.24.10.0322","DOIUrl":null,"url":null,"abstract":"Objective: To investigate a point-of-care viscoelastic coagulation monitor (VCM Vet; Entegrion Inc), compare the results to thromboelastography (TEG), and quantify traditional hemostatic analytes in southern white rhinoceros (Ceratotherium simum simum).Methods: 9 female rhinoceros (4 juveniles [2 to 3 years old], 4 adults [16 to 34 years old], and 1 geriatric [54 years old]) at the North Carolina Zoo were enrolled. Whole blood was collected using trained voluntary behavior and analyzed in duplicate via both VCM Vet and kaolin-activated TEG within 4 minutes or at 30 minutes following collection, respectively. Citrated plasma was used for ancillary coagulation testing.Results: Both analyses generated quantifiable clotting reactions with variables (median [range]) related to clot formation rate (TEG: R = 9.4 minutes [5.1 to 10.8], K = 2.0 minutes [1.4 to 3.0], α angle = 66° [41° to 73°]; VCM Vet: CT = 882 seconds [758 to 1,252], CFT = 416 seconds [200 to 980], α = 24° [11° to 43°]), clot strength (TEG: MA = 71 mm [64 to 79], G = 11.9 kilodynes/s [9.0 to 18.9]; VCM Vet: MCF = 49 units [34 to 53]), and clot lysis (TEG: LY30 = 0.9% [0% to 1.7%], LY60 = 3.2% [0.9% to 4.9%]; VCM Vet: Li30 = 100% [99% to 100%], Li45 = 98% [93% to 100%]) recorded. Additional testing (median [range]) included D-dimer (221 ng/mL [138 to 577]), prothrombin time (21.4 seconds [19.6 to 23.7]), activated partial thromboplastin time (24.8 seconds [22.5 to 27.4]), and fibrinogen (336 mg/dL [280 to 429]).Conclusions: Tracings generated by VCM Vet and TEG were clinically similar, and there was visual agreement and minimal difference between quantitative variables for duplicate tests.Clinical relevance: VCM Vet is a user-friendly, portable device that demonstrates promise for assessing coagulation in southern white rhinoceros.","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-9"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.10.0322","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To investigate a point-of-care viscoelastic coagulation monitor (VCM Vet; Entegrion Inc), compare the results to thromboelastography (TEG), and quantify traditional hemostatic analytes in southern white rhinoceros (Ceratotherium simum simum).

Methods: 9 female rhinoceros (4 juveniles [2 to 3 years old], 4 adults [16 to 34 years old], and 1 geriatric [54 years old]) at the North Carolina Zoo were enrolled. Whole blood was collected using trained voluntary behavior and analyzed in duplicate via both VCM Vet and kaolin-activated TEG within 4 minutes or at 30 minutes following collection, respectively. Citrated plasma was used for ancillary coagulation testing.

Results: Both analyses generated quantifiable clotting reactions with variables (median [range]) related to clot formation rate (TEG: R = 9.4 minutes [5.1 to 10.8], K = 2.0 minutes [1.4 to 3.0], α angle = 66° [41° to 73°]; VCM Vet: CT = 882 seconds [758 to 1,252], CFT = 416 seconds [200 to 980], α = 24° [11° to 43°]), clot strength (TEG: MA = 71 mm [64 to 79], G = 11.9 kilodynes/s [9.0 to 18.9]; VCM Vet: MCF = 49 units [34 to 53]), and clot lysis (TEG: LY30 = 0.9% [0% to 1.7%], LY60 = 3.2% [0.9% to 4.9%]; VCM Vet: Li30 = 100% [99% to 100%], Li45 = 98% [93% to 100%]) recorded. Additional testing (median [range]) included D-dimer (221 ng/mL [138 to 577]), prothrombin time (21.4 seconds [19.6 to 23.7]), activated partial thromboplastin time (24.8 seconds [22.5 to 27.4]), and fibrinogen (336 mg/dL [280 to 429]).

Conclusions: Tracings generated by VCM Vet and TEG were clinically similar, and there was visual agreement and minimal difference between quantitative variables for duplicate tests.

Clinical relevance: VCM Vet is a user-friendly, portable device that demonstrates promise for assessing coagulation in southern white rhinoceros.

查看原文本刊更多论文

9头雌性南白犀牛（Ceratotherium simum simum）现场粘弹性凝血监护仪的研究及其与血栓弹性成像的比较。

目的：研究现场粘弹性凝血监护仪(VCM Vet；将结果与血栓弹性成像（TEG）进行比较，并量化南方白犀牛（Ceratotherium simum simum）的传统止血分析物。方法：选取北卡罗来纳动物园9头雌性犀牛（4头幼年犀牛[2 ~ 3岁]，4头成年犀牛[16 ~ 34岁]，1头老年犀牛[54岁]）。采用经过训练的自愿行为采集全血，并在采集后4分钟或30分钟内分别通过VCM Vet和高岭土活化TEG进行两份分析。柠檬酸血浆用于辅助凝血试验。结果：两种分析都产生了可量化的凝血反应，其变量（中位数[范围]）与凝块形成率相关(TEG: R = 9.4分钟[5.1至10.8],K = 2.0分钟[1.4至3.0]，α角= 66°[41°至73°]；VCM Vet: CT = 882秒[758 ~ 1,252],CFT = 416秒[200 ~ 980]，α = 24°[11°~ 43°])，凝块强度(TEG: MA = 71 mm [64 ~ 79], G = 11.9千焦/秒[9.0 ~ 18.9]；VCM Vet: MCF = 49单位[34 ~ 53])，凝块溶解(TEG: LY30 = 0.9% [0% ~ 1.7%], LY60 = 3.2% [0.9% ~ 4.9%]；VCM Vet: Li30 = 100% [99% ~ 100%], Li45 = 98%[93% ~ 100%])记录。其他检测（中位数[范围]）包括d -二聚体（221 ng/mL[138至577]）、凝血酶原时间（21.4秒[19.6至23.7]）、活化的部分凝血活素时间（24.8秒[22.5至27.4]）和纤维蛋白原（336 mg/dL[280至429]）。结论：VCM Vet和TEG所产生的示踪在临床上相似，重复试验的定量变量之间存在视觉一致性和最小差异。临床意义：VCM Vet是一种用户友好的便携式设备，可用于评估南部白犀牛的凝血。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.