Cerebral ischemia-reperfusion induces the expression of phoenixin receptor (GPR173) and adult neurogenesis marker proteins in the rat striatum.

IF 1.5 4区医学 Q4 NEUROSCIENCES

Brain injury Pub Date : 2025-05-12 Epub Date: 2024-12-29 DOI:10.1080/02699052.2024.2443004

Kinga Mordecka-Chamera, Artur Pałasz, Aleksandra Suszka-Świtek, Katarzyna Bogus, Władysław Skałba, Aneta Piwowarczyk-Nowak, John J Worthington, Marta Pukowiec, Veerta Sharma, Łukasz Filipczyk

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引用次数: 0

Abstract

Objective: Brain ischemia is considered an extremely potent stress factor at the cellular and molecular level which may lead to massive neuronal death. Alternatively, short brain ischemia and reperfusion (I/R) can actually stimulate neurogenesis, angiogenesis and peptidergic signaling. There is little known about the potential effect of I/R on brain expression of the novel neuropeptide; phoenixin (PNX) and its receptor GPR173.

Methods: The study was carried out on adult male Wistar rats divided into seven groups: control, sham operation and 5 ischemic experimental groups across the time course of reperfusion. We examined mRNA and protein expression of GPR173 and neurogenesis markers Musashi-1, doublecortin (DCX), and Sox-2 in the striatum.

Results: GPR-173 positive cells were found only in the ischemic hemisphere, where Musashi-1, DCX and Sox-2-positive cells were also observed. Gene expression analysis also showed a significant increase of GPR-173 mRNA level in the I/R striatum in comparison with the control one. Results confirm previous findings suggesting that I/R stimulates adult neurogenesis in the striatum and affects peptidergic signaling in this structure.

Conclusions: A very fast occurence of GPR-173 expression revealed in the striatum may potentially be exclusively related to neuroprotective neurochemical changes that occur in this region after I/R.

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脑缺血再灌注诱导大鼠纹状体中凤凰素受体（GPR173）和成体神经发生标志蛋白的表达。

目的：脑缺血在细胞和分子水平上被认为是一种非常有效的应激因素，可导致大量神经元死亡。另外，脑短缺血再灌注（I/R）实际上可以刺激神经发生、血管生成和肽能信号传导。目前对I/R对这种新型神经肽脑表达的潜在影响知之甚少；凤凰素（PNX）及其受体GPR173。方法：将成年雄性Wistar大鼠按再灌注时间分为对照组、假手术组和5个缺血实验组。我们检测了纹状体中GPR173和神经发生标记物Musashi-1、双皮质素（DCX）和Sox-2的mRNA和蛋白表达。结果：GPR-173阳性细胞仅出现在缺血半球，同时也出现Musashi-1、DCX和sox -2阳性细胞。基因表达分析也显示，与对照相比，I/R纹状体中GPR-173 mRNA水平显著升高。结果证实了先前的发现，即I/R刺激纹状体的成人神经发生并影响该结构的肽能信号传导。结论：纹状体中GPR-173的快速表达可能只与I/R后该区域发生的神经保护性神经化学变化有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain injury 医学-康复医学

CiteScore

3.50

自引率

5.30%

发文量

148

审稿时长

12 months

期刊介绍： Brain Injury publishes critical information relating to research and clinical practice, adult and pediatric populations. The journal covers a full range of relevant topics relating to clinical, translational, and basic science research. Manuscripts address emergency and acute medical care, acute and post-acute rehabilitation, family and vocational issues, and long-term supports. Coverage includes assessment and interventions for functional, communication, neurological and psychological disorders.