Protective Effect of Amifostine on Radiotherapy-Applied Cardiovascular Tissue.

IF 1.5 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Anatolian Journal of Cardiology Pub Date : 2025-04-21 DOI:10.14744/AnatolJCardiol.2025.4996

Gökay Taylan, Murat Çaloğlu, Vuslat Yürüt Çaloğlu, Tülin Yalta, Nurettin Aydoğdu, Kenan Yalta, Meryem Aktoz

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Abstract

Background: The present study evaluates the protective effect of amifostine (AMI) on acute toxicity in large vessels and the heart in rats with radiotherapy (RT) applied to the thorax.

Methods: Twenty-one Wistar albino rats were randomly assigned to 3 groups: Alone RT (n = 7), amifostine plus RT (AMI+RT, n = 7), and control (n = 7) groups. The rats in the RT and AMI+RT groups received a single dose of 20 Gy radiation to the entire thorax. Prior to irradiation, AMI was administered intraperitoneally at a dose of 200 mg/kg, 30 minutes before the procedure. Five days after irradiation, the levels of p53, CD68, and COX in the vascular tissue (aorta) were measured, along with the levels of malondialdehyde (MDA) and glutathione (GSH) in the aortic and heart tissues.

Results: The results showed that the level of MDA significantly increased after irradiation, but GSH levels did not change (P < .001 and P = 0.138). Malondialdehyde levels were significantly reduced by AMI, and GSH levels increased (P = .031 and P = .007). When comparing the control group with AMI + RT, MDA and glutathione levels were similar (P = .314 and P = .136). Histopathological evaluation revealed increased cellular inflammation (P = .002) and vascular damage (P = .015) in aortic tissue after thoracic RT irradiation, but no difference in terms of myofibrosis (P = .901) in heart tissue.

Conclusion: AMI has a radioprotective and antioxidant effect against RT-induced cardiovascular toxicity.

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氨磷汀对放疗后心血管组织的保护作用。

背景：本研究评价氨磷汀（AMI）对大鼠胸腔放射治疗（RT）大血管和心脏急性毒性的保护作用。方法：将21只Wistar白化大鼠随机分为单独RT组（n = 7）、氨磷汀联合RT组（AMI+RT, n = 7）和对照组（n = 7）。RT组和AMI+RT组大鼠全胸接受单剂量20 Gy辐射。在照射前，AMI在手术前30分钟以200 mg/kg的剂量腹腔注射。照射5天后，测量血管组织（主动脉）中p53、CD68和COX水平，以及主动脉和心脏组织中丙二醛（MDA）和谷胱甘肽（GSH）水平。结果：照射后大鼠血清MDA水平明显升高，GSH水平无明显变化（P < 0.001, P = 0.138）。AMI组丙二醛水平显著降低，GSH水平升高（P = 0.031和P = 0.007）。AMI + RT组与对照组比较，MDA和谷胱甘肽水平相似（P = 0.314和P = 0.136）。组织病理学评估显示，经胸腔放射治疗后主动脉组织细胞炎症（P = 0.002）和血管损伤（P = 0.015）增加，但心脏组织肌纤维化（P = 0.901）无差异。结论：AMI对rt诱导的心血管毒性具有放射防护和抗氧化作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anatolian Journal of Cardiology CARDIAC & CARDIOVASCULAR SYSTEMS-

CiteScore

2.30

自引率

7.70%

发文量

270

审稿时长

12 weeks

期刊介绍： The Anatolian Journal of Cardiology is an international monthly periodical on cardiology published on independent, unbiased, double-blinded and peer-review principles. The journal’s publication language is English. The Anatolian Journal of Cardiology aims to publish qualified and original clinical, experimental and basic research on cardiology at the international level. The journal’s scope also covers editorial comments, reviews of innovations in medical education and practice, case reports, original images, scientific letters, educational articles, letters to the editor, articles on publication ethics, diagnostic puzzles, and issues in social cardiology. The target readership includes academic members, specialists, residents, and general practitioners working in the fields of adult cardiology, pediatric cardiology, cardiovascular surgery and internal medicine.