Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2025-04-30 DOI:10.3390/ani15091280

Mingyue Shi, Wenxia Li, Shuai Yang, Qipin Lv, Jingxian Yang, Di Sun, Guanqing Yang, Yan Zhao, Wanfeng Zhang, Meng Li, Yang Yang, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li, Guoqing Cao

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引用次数: 0

Abstract

Chinese native pig breeds exhibit unique advantages over Western pig breeds, but the specific lipid metabolism mechanisms remain unclear. The phenotypic characteristics of Mashen (MS) pigs and Duroc × (Landrace × Yorkshire) (DLY) pigs are studied. The results show that MS pigs exhibit higher intramuscular fat (IMF) content. The area of adipocytes of MS pigs is significantly greater than that in DLY pigs (p < 0.01). Lipidomics analysis reveals distinct profiles in the upper layer of backfat (ULB), leaf lard (LL), greater omentum (GOM), and IMF, with MS pigs showing higher polyunsaturated fatty acids (PUFAs) in ULB, LL, and GOM. Key differential lipids identified in the two pig breeds include the following triglycerides (TGs) and phosphatidylcholines (PC): TG(16:1_18:1_18:3), TG(18:1_18:2_18:3), TG(18:3_18:2_18:2), PC(18:0_18:1), and PC(18:0_18:2). Weighted gene co-expression network analysis (WGCNA) reveals lipid molecules associated with serum biochemical indices. Transcriptomics analysis highlights 1944 differentially expressed genes between the MS-ULB and DLY-ULB. Notably, multiple genes from the cytochrome P450 family (CYP2E1, CYP4A24, CYP2J2), along with PLA2G2D, PLA2G4A, and multiple PCs, are associated with the metabolism of arachidonic acids and linoleic acids. PLA2G2D and PLA2G4A are also involved in the metabolism of α-linolenic acids. This comprehensive analysis provides essential information for breeding strategies and meat quality improvement.

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综合多组织脂质组学和转录组学揭示马胜猪和杜洛克×（长×大）猪脂质组成差异。

中国本土猪品种比西方猪品种表现出独特的优势，但具体的脂质代谢机制尚不清楚。研究了马胜（MS）猪和杜×长×大（DLY）猪的表型特征。结果表明，MS猪肌内脂肪（IMF）含量较高。MS猪的脂肪细胞面积显著大于DLY猪（p < 0.01）。脂质组学分析显示，在背膘（ULB）、叶脂（LL）、大网膜（GOM）和IMF的上层有不同的分布，而MS猪在ULB、LL和GOM中显示出更高的多不饱和脂肪酸（PUFAs）。在两个猪品种中鉴定出的关键差异脂质包括甘油三酯（TG）和磷脂酰胆碱（PC）： TG（16:1_18: 18:3）、TG（18:1_18:2_18:3）、TG（18:3_18:2_18:2）、PC（18:0_18:1）和PC（18:0_18:2）。加权基因共表达网络分析（WGCNA）揭示了脂质分子与血清生化指标的相关性。转录组学分析显示MS-ULB和DLY-ULB之间有1944个差异表达基因。值得注意的是，来自细胞色素P450家族的多个基因（CYP2E1、CYP4A24、CYP2J2）、PLA2G2D、PLA2G4A和多个pc与花生四烯酸和亚油酸的代谢有关。PLA2G2D和PLA2G4A也参与α-亚麻酸的代谢。这一综合分析为育种策略和肉质改良提供了重要信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).