[Expression and regulatory mechanism of miR-34a in neonatal rat model of bron-chopulmonary dysplasia induced by hyperoxia].

Q3 Medicine
北京大学学报(医学版) Pub Date : 2025-04-18
Mengyue Huo, Hua Mei, Yuheng Zhang, Yanbo Zhang, Chunli Liu
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引用次数: 0

Abstract

Objective: To investigate the expression and possible regulatory mechanism of miR-34a in the lung tissue of neonatal rat model of bronchopulmonary dysplasia (BPD) induced by hyperoxia.

Methods: In the study, 80 newborn SD rats were randomly divided into hyperoxia group (FiO2=60%) and air group (FiO2=21%) within 2 hours after birth, 40 rats per group. Lung tissue samples of the SD rats in each group were extracted on the 1st, 7th, 14th and 21st days after birth, and the pathological changes of lung tissue were observed under light microscope after HE staining. The number of radial alveolar counts (RAC) and the mean alveolar diameter (MAD) and the thickness of alveolar septal thickness (AST) were measured to evaluate the development of alveoli. Real-time fluorescence quantitative PCR was used to detect the expression of miR-34a, angiopoietin-1 (Ang-1) and tyrosine kinase receptor-2 (Tie-2) in lung tissue of rats in hyperoxia group and air group at different time points. Enzyme-linked immunosorbent assay (ELISA) was used to detect the proteins expression of Ang-1 and Tie-2 in the lung tissues of the two groups at different time points.

Results: The weight of rats in the hyperoxia group on the 7th, 14th and 21st days after birth was significantly lower than that in the air group (P all < 0.05). With the prolongation of oxygen exposure, the number of alveoli decreased, the volume increased, the structure simplified, the alveolar cavity enlarged obviously and the alveolar septum thickened in the hyperoxia group. On the 7th, 14th and 21st days after birth, the RAC in the hyperoxia group was significantly lower than that in the air group (P all < 0.05). Compared with the air group, MAD and AST increased significantly on the 7th, 14th and 21st days after birth in the hyperoxia group, and the difference was statistically significant (P all < 0.05). The expression level of miR-34a in lung tissue of hyperoxia group was significantly higher than that of air group on the 7th, 14th and 21st days after birth, and the difference was statistically significant (P all < 0.05). Compared with the air group at the same time point, the expression levels of Ang-1 and Tie-2 mRNA and protein in the hyperoxia group were lower than those in the air group on the 14th and 21st days after birth (P all < 0.05).

Conclusion: The new BPD model of newborn SD rats can be successfully established by continuous exposure to 60% hyperoxia. The expression of miR-34a was up-regulated in the lung tissue of the new BPD model of neonatal rats. MiR-34a may play an important role in the occurrence and development of BPD by regulating Ang-1/Tie-2 signal pathway.

[miR-34a在新生儿大鼠高氧肺发育不良模型中的表达及调控机制]。
目的:探讨miR-34a在高氧诱导的新生大鼠支气管肺发育不良(BPD)模型肺组织中的表达及其可能的调控机制。方法:将80只新生SD大鼠在出生后2 h内随机分为高氧组(FiO2=60%)和空气组(FiO2=21%),每组40只。各组SD大鼠分别于出生后第1、7、14、21天提取肺组织标本,经HE染色,光镜下观察肺组织病理变化。测定桡骨肺泡计数(RAC)、平均肺泡直径(MAD)和肺泡间隔厚度(AST),评价肺泡的发育情况。采用实时荧光定量PCR检测不同时间点高氧组和空气组大鼠肺组织中miR-34a、血管生成素-1 (ang1)和酪氨酸激酶受体-2 (Tie-2)的表达。采用酶联免疫吸附法(ELISA)检测两组不同时间点肺组织中Ang-1、Tie-2蛋白的表达情况。结果:高氧组大鼠出生后第7、14、21天体重均显著低于空气组(P均< 0.05)。随着氧暴露时间的延长,高氧组肺泡数量减少,体积增大,结构简化,肺泡腔明显增大,肺泡隔增厚。在出生后第7、14、21天,高氧组RAC显著低于空气组(P均< 0.05)。与空气组比较,高氧组MAD、AST在出生后第7、14、21天显著升高,差异均有统计学意义(P均< 0.05)。高氧组在出生后第7、14、21天肺组织中miR-34a的表达水平显著高于空气组,差异均有统计学意义(P均< 0.05)。与空气组在同一时间点比较,高氧组在出生后第14、21天Ang-1、Tie-2 mRNA及蛋白表达水平均低于空气组(P均< 0.05)。结论:持续60%高氧可成功建立新生SD大鼠BPD模型。miR-34a在新生BPD模型大鼠肺组织中表达上调。MiR-34a可能通过调节Ang-1/Tie-2信号通路在BPD的发生发展中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
北京大学学报(医学版)
北京大学学报(医学版) Medicine-Medicine (all)
CiteScore
0.80
自引率
0.00%
发文量
9815
期刊介绍: Beijing Da Xue Xue Bao Yi Xue Ban / Journal of Peking University (Health Sciences), established in 1959, is a national academic journal sponsored by Peking University, and its former name is Journal of Beijing Medical University. The coverage of the Journal includes basic medical sciences, clinical medicine, oral medicine, surgery, public health and epidemiology, pharmacology and pharmacy. Over the last few years, the Journal has published articles and reports covering major topics in the different special issues (e.g. research on disease genome, theory of drug withdrawal, mechanism and prevention of cardiovascular and cerebrovascular diseases, stomatology, orthopaedic, public health, urology and reproductive medicine). All the topics involve latest advances in medical sciences, hot topics in specific specialties, and prevention and treatment of major diseases. The Journal has been indexed and abstracted by PubMed Central (PMC), MEDLINE/PubMed, EBSCO, Embase, Scopus, Chemical Abstracts (CA), Western Pacific Region Index Medicus (WPR), JSTChina, and almost all the Chinese sciences and technical index systems, including Chinese Science and Technology Paper Citation Database (CSTPCD), Chinese Science Citation Database (CSCD), China BioMedical Bibliographic Database (CBM), CMCI, Chinese Biological Abstracts, China National Academic Magazine Data-Base (CNKI), Wanfang Data (ChinaInfo), etc.
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