[In vitro study of using single cone obturation technique in artificial canals with an isthmus].

Q3 Medicine
北京大学学报(医学版) Pub Date : 2025-04-18
Yuqi Ma, Yuhong Liang
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引用次数: 0

Abstract

Objective: To evaluate the filling quality of single cone obturation in root canal model with irregular structure (Hus&Kim Ⅴ, Yin Ⅱ-type isthmus) which established by 3D printing technology using slices and radiographic methods, in order to provide reference for clinical practice.

Methods: (1) Extracted fresh premolars with Hus&Kim Ⅴ and Yin-type Ⅱ isthmus were collected and scanned by cone-beam computed tomography (CBCT), then standard root canal models were designed and printed. Rhodamine B staining and bias fitting were used to verify the availability of the models. (2) 30 root canal models were randomly divided into 3 groups according to different filling methods (n=10).

Control group: vertical compaction obturation; Experimental group 1: single cone obturation with 0.06-taper cone (30#); Experimental group 2: single cone obturation with 0.04-taper cone (35#), GuttaFlow 2 as canal sealers. Slices were taken at 2, 4, 6, and 8 mm from the root apex in the direction perpendicular to the long axis of the root and observed under a stereomicroscope to calculate the percentage of filling area (PAV), percentage of gutta-percha-filled area (PGFA), percentage of sealer filled area (PSFA). (3) On the basis of the above results, two groups (n=4) were selected to further analyze the filling quality by micro-computed tomography (Micro-CT), the filling volume of main root canal and the isthmus were obtained, and the percentage of filling volume (PFV) was calculated. Two-way ANOVA was used to evaluate the differences between the groups, and Tukey' s multiple comparison was used to compare the data between the groups and within the groups.

Results: (1) Rhodamine B staining solution could overflow the apical foramen, and the main root canal system and the isthmus area were stained, showed no remnants of support material. The 3D standard deviation of the printed model data was 0.03 mm, and the average fitting distance was 0.02 mm. (2) The PFA of the two experimental groups were both significantly lower than that of the control group (F=45.04, P < 0.01). There was no statistical difference of the PFA at apical 2 and 4 mm between the two experimental groups (P>0.01), but at the middle and coronal portions of the root canal (6, 8 mm), the PFA of the experimental group 1 was higher than that of the experimental group 2 (P < 0.01). PFA in the apical 2, 4 mm of the two experimental groups were both lower than that in the middle and coronal portions 6, 8 mm of the canal (P < 0.01). There was no difference in the PGFA and PSFA between the two experimental groups at the apical 2, 4 mm (F=2.383, P>0.01). (3) The results of Micro-CT showed that the PFV of the experimental group 1 was statistically different with the control group (F=47.33, P < 0.01). The PFV of the experimental group 1 was 54.33%±4.35% in the isthmus and 78.31%±4.21% in the main root canal, which were both lower than the PFV of the control group of 76.48%±4.89% (isthmus) and 86.90%±3.29% (main root canal, P < 0.01). The PFV of the main root canal in the experimental group 1 was higher than that in the isthmus (P < 0.01), while there was no difference between the isthmus and the main root canal in the control group (P>0.01).

Conclusion: In the irregular root canal structure with isthmus, using large-taper gutta-percha can improve the filling quality of the middle and upper part of the canal, but the percentage of filling volume in the isthmus is lower than that of the main canal, and more technical improvements are needed.

[单锥封闭技术在峡部人工管道中的体外研究]。
目的:评价3D打印技术建立的不规则结构根管模型(Hus&KimⅤ,YinⅡ型峡部)单锥充填质量,为临床实践提供参考。方法:(1)采集提取的新鲜前磨牙(Hus&KimⅤ型和yin型Ⅱ型)峡部,采用锥束计算机断层扫描(CBCT),设计并打印标准根管模型。罗丹明B染色和偏倚拟合用于验证模型的可用性。(2) 30个根管模型根据充填方式的不同随机分为3组(n=10)。对照组:垂直压实封闭;实验组1:单锥封闭,锥度为0.06 (30#);实验组2:单锥封闭,采用0.04锥(35#),GuttaFlow 2作为根管封闭剂。垂直于根长轴方向,在离根尖2、4、6、8 mm处取片,在体视显微镜下观察,计算填充面积百分比(PAV)、胶-高胶填充面积百分比(PGFA)、密封胶填充面积百分比(PSFA)。(3)在以上结果的基础上,选择两组(n=4)进一步通过Micro-CT (Micro-CT)分析充填质量,获得主根管和峡部的充填体积,并计算充填体积百分比(PFV)。采用双因素方差分析评价组间差异,采用Tukey多重比较比较组间及组内数据。结果:(1)罗丹明B染色液可溢出根尖孔,主根管系统及峡部区域均有染色,未见支撑物质残留。打印模型数据的3D标准差为0.03 mm,平均拟合距离为0.02 mm。(2)两实验组的PFA均显著低于对照组(F=45.04, P < 0.01)。两组根管根尖2、4 mm处的PFA比较,差异无统计学意义(P < 0.01),但在根管中、冠状段(6、8 mm),实验组1的PFA高于实验组2 (P < 0.01)。两试验组根尖2、4 mm的PFA均低于根管中、冠状6、8 mm的PFA (P < 0.01)。两组患者在根尖2,4 mm处的PGFA和PSFA差异无统计学意义(F=2.383, P < 0.01)。(3) Micro-CT结果显示,实验组1与对照组PFV差异有统计学意义(F=47.33, P < 0.01)。试验1组峡部PFV为54.33%±4.35%,主根管为78.31%±4.21%,均低于对照组峡部76.48%±4.89%和主根管86.90%±3.29%,P < 0.01。实验1组主根管的PFV高于峡部(P < 0.01),而对照组峡部与主根管无显著差异(P < 0.01)。结论:对于有峡部的根管结构不规则的根管,使用大锥度杜仲胶可以改善根管中上段的充填质量,但峡部的充填体积比例低于主根管,还需要进一步的技术改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
北京大学学报(医学版)
北京大学学报(医学版) Medicine-Medicine (all)
CiteScore
0.80
自引率
0.00%
发文量
9815
期刊介绍: Beijing Da Xue Xue Bao Yi Xue Ban / Journal of Peking University (Health Sciences), established in 1959, is a national academic journal sponsored by Peking University, and its former name is Journal of Beijing Medical University. The coverage of the Journal includes basic medical sciences, clinical medicine, oral medicine, surgery, public health and epidemiology, pharmacology and pharmacy. Over the last few years, the Journal has published articles and reports covering major topics in the different special issues (e.g. research on disease genome, theory of drug withdrawal, mechanism and prevention of cardiovascular and cerebrovascular diseases, stomatology, orthopaedic, public health, urology and reproductive medicine). All the topics involve latest advances in medical sciences, hot topics in specific specialties, and prevention and treatment of major diseases. The Journal has been indexed and abstracted by PubMed Central (PMC), MEDLINE/PubMed, EBSCO, Embase, Scopus, Chemical Abstracts (CA), Western Pacific Region Index Medicus (WPR), JSTChina, and almost all the Chinese sciences and technical index systems, including Chinese Science and Technology Paper Citation Database (CSTPCD), Chinese Science Citation Database (CSCD), China BioMedical Bibliographic Database (CBM), CMCI, Chinese Biological Abstracts, China National Academic Magazine Data-Base (CNKI), Wanfang Data (ChinaInfo), etc.
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