Recombinant fusion protein LipL41-OmpL1 as a Potential Candidate for a Cost-effective Vaccine against Leptospirosis.

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.32592/ARI.2024.79.5.1099
M Eghbal, P Khaki, F Ghandehari, M Taghizadeh, M Tebianian
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引用次数: 0

Abstract

Leptospirosis represents a significant threat to the health of both humans and animals. It is a disease caused by pathogenic Leptospira. The early detection of pathogenic Leptospira is an effective method for preventing a multitude of potential complications. The protected outer membrane protein (OMP) of pathogenic Leptospira, LipL41-OmpL1, was inserted into E. coli bacteria using different software for the amino acid sequence of OmpL1 and LipL41. This was done to design a recombinant fusion protein, which was then expressed to investigate immunogenicity. The selected genes were propagated and cloned as a fusion in a PET32a+ plasmid vector and expressed by Escherichia coli strain S (DE3) via a heat shock method. The evaluation was conducted using the BALB/c mouse as the laboratory animal model. The recombinant LipL41-OmpL1 protein was confirmed using the urea purification method and western blot, and its immunogenicity was evaluated by measuring the high humoral immune stimulation and antibody secretion in BALB/c mice by the ELISA method. The findings demonstrated that the animals that received both the OmpL1 and LipL41 proteins exhibited 85% immunogenicity, whereas the control group that did not receive the fusion protein demonstrated only 25% immunogenicity (P<0.001). Moreover, no evidence of infection was identified in recipients of the OmpL1-LipL41 fusion protein, indicating that this protein is safe for use. The protective effects of immunization with OmpL1 and LipL41 were synergistic, as no significant levels of protection were observed in animals immunized with OmpL1 or LipL41 alone. In conclusion, this study underscores the potential of a recombinant OmpL1 and LipL41 fusion protein as a promising avenue for research in the development of vaccines and ELISA diagnostic kits for the prevention and rapid diagnosis of leptospirosis.

重组融合蛋白LipL41-OmpL1作为具有成本效益的钩端螺旋体病疫苗的潜在候选物
钩端螺旋体病对人类和动物的健康构成重大威胁。它是一种由致病性钩端螺旋体引起的疾病。早期发现致病性钩端螺旋体是预防多种潜在并发症的有效方法。将致病性钩端螺旋体保护外膜蛋白(OMP) LipL41-OmpL1用不同的软件插入大肠杆菌中,测定其氨基酸序列。这样做是为了设计一个重组融合蛋白,然后表达以研究免疫原性。选择的基因在PET32a+质粒载体上进行扩增和融合克隆,用大肠杆菌S (DE3)菌株热休克法表达。以BALB/c小鼠为实验动物模型进行评价。重组LipL41-OmpL1蛋白通过尿素纯化法和western blot证实,并通过ELISA法测定BALB/c小鼠的高体液免疫刺激和抗体分泌来评价其免疫原性。结果表明,同时接受OmpL1和LipL41蛋白的动物表现出85%的免疫原性,而未接受融合蛋白的对照组仅表现出25%的免疫原性(P
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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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