NT-OmpA Bioemulsifier: Structural Insights and Polysaccharide-Mediated Enhancement of Its Emulsifying Performance.

IF 3 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

ChemPlusChem Pub Date : 2025-04-22 DOI:10.1002/cplu.202400774

Naeema Mohseni Sani, Mahbubeh Talaee, Fatemeh Saadati, Faranak Ashoori, Javad Zamani, Hossein Shahbani Zahiri, Hojatollah Vali, Kambiz Akbari Noghabi

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引用次数: 0

Abstract

Our recent studies found that NT-OmpA (N-terminally truncated SA01-OmpA) has distinct advantages over full-length SA01-OmpA, including non-cytotoxicity, enhanced emulsifying capacity, and higher production yield. This study builds upon prior research by investigating the emulsifying properties of NT-OmpA under various environmental conditions, such as temperature, salinity, and pH. Molecular dynamics (MD) simulations and circular dichroism (CD) spectroscopy revealed significant alterations in the secondary structure of NT-OmpA under extreme conditions, specifically at alkaline pH (pH 11) and elevated temperatures (90°C). Circular dichroism (CD) spectroscopy showed a significant decrease in alpha-helical content and increased beta-sheet structures, validating NT-OmpA's pH stability. We investigated NT-OmpA's effectiveness when combined with various polysaccharides to enhance its functional properties. Dextran (emulsification index of 76.94 ± 0.05 %) exhibited superior emulsifying activity compared to glucose, agarose, starch, and NT-OmpA alone. NT-OmpA's emulsifying characteristics are considerably improved by dextran, making this combination a promising next-generation emulsifier. These findings underscore the structural resilience and functional adaptability of NT-OmpA, especially in challenging environmental conditions, and highlight its potential in industrial applications requiring robust emulsifying agents.

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NT-OmpA生物乳化剂：结构和多糖介导的乳化性能增强。

我们最近的研究发现NT-OmpA （n端截断的SA01-OmpA）比全长SA01-OmpA具有明显的优势，包括无细胞毒性，增强的乳化能力和更高的产量。本研究基于之前的研究，研究了NT-OmpA在不同环境条件下的乳化性能，如温度、盐度和pH。分子动力学（MD）模拟和圆二色性（CD）光谱显示，在极端条件下，特别是在碱性pH （pH 11）和高温（90°C）下，NT-OmpA的二级结构发生了显著变化。圆二色性（CD）光谱显示，α -螺旋含量显著降低，β -片结构增加，验证了NT-OmpA的pH稳定性。我们研究了NT-OmpA与各种多糖联合使用以增强其功能特性的有效性。与葡萄糖、琼脂糖、淀粉和NT-OmpA单独相比，右旋糖酐的乳化指数为76.94±0.05%。右旋糖酐大大改善了NT-OmpA的乳化特性，使这种组合成为有前途的下一代乳化剂。这些发现强调了NT-OmpA的结构弹性和功能适应性，特别是在具有挑战性的环境条件下，并突出了其在需要强效乳化剂的工业应用中的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ChemPlusChem CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

5.90

自引率

0.00%

发文量

200

审稿时长

1 months

期刊介绍： ChemPlusChem is a peer-reviewed, general chemistry journal that brings readers the very best in multidisciplinary research centering on chemistry. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. Fully comprehensive in its scope, ChemPlusChem publishes articles covering new results from at least two different aspects (subfields) of chemistry or one of chemistry and one of another scientific discipline (one chemistry topic plus another one, hence the title ChemPlusChem). All suitable submissions undergo balanced peer review by experts in the field to ensure the highest quality, originality, relevance, significance, and validity.