Molecular identification, antibiotic susceptibility, and biofilm formation of airborne bacteria.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Malik Asif Hussain, Mubashir Zafar, Yusuf Saleem Khan, Kamaleldin B Said, Sadaf Anwar, Mohd Saeed, Mohammad A Abdulhakeem, Mejdi Snoussi, Mohd Adnan Kausar
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Abstract

Pathogenic bacterial communities present in urban green spaces significantly affect human health, particularly for immunocompromised populations. The diverse range of pathogenic bacteria found in these areas poses substantial management challenges because of their high prevalence of antibiotic resistance, which can be life-threatening, particularly for immunocompromised individuals, including older adults and children. This study identified airborne bacterial species from 14 natural parks in the Hail region of the Kingdom of Saudi Arabia. Bacterial colonies isolated on blood agar plates were purified and characterised based on their morphological traits and their ability to secrete various virulence factors. A total of 28 distinct airborne bacterial species were isolated and purified. Antibiotic susceptibility tests revealed high resistance to fosfomycin (41.17%), ampicillin (17.64%), tetracycline (17.64%), and gentamicin (11.76%). Biofilm formation was evaluated qualitatively by slime production and quantitatively by crystal violet technique. The results revealed that 41.17% of the tested strains were non biofilm producers on polystyrene surfaces, 17.64% were weak biofilm formers, and 23.52% exhibited moderate biofilm formation. Notably, six strains exhibited strong biofilm-forming capabilities. Additionally, two bacteria from the Arthrobacter genus (A. crystallopoietes and A. saudimassiliensis) were identified. These findings provide valuable insights into the microbial composition of natural parks in the Hail region and highlight effective management strategies to mitigate health risks.

空气传播细菌的分子鉴定、抗生素敏感性和生物膜形成。
城市绿地中存在的致病菌群落严重影响人类健康,特别是对免疫功能低下的人群。在这些地区发现的各种致病菌构成了重大的管理挑战,因为它们普遍存在抗生素耐药性,可能危及生命,特别是对免疫功能低下的个体,包括老年人和儿童。本研究鉴定了来自沙特阿拉伯王国海尔地区14个自然公园的空气传播细菌种类。对血琼脂平板上分离的细菌菌落进行了纯化,并根据其形态特征和分泌各种毒力因子的能力进行了鉴定。共分离纯化了28种不同的空气传播细菌。药敏试验显示对磷霉素(41.17%)、氨苄西林(17.64%)、四环素(17.64%)和庆大霉素(11.76%)耐药。用黏液法定性评价生物膜的形成,结晶紫法定量评价生物膜的形成。结果表明,41.17%的菌株在聚苯乙烯表面不形成生物膜,17.64%的菌株形成弱生物膜,23.52%的菌株形成中等生物膜。值得注意的是,6株菌株表现出较强的生物膜形成能力。此外,还鉴定出两种节肢菌属细菌(A. crystallopoietes和A. saudimassiliensis)。这些发现为了解冰雹地区自然公园的微生物组成提供了有价值的见解,并强调了减轻健康风险的有效管理策略。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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