Malik Asif Hussain, Mubashir Zafar, Yusuf Saleem Khan, Kamaleldin B Said, Sadaf Anwar, Mohd Saeed, Mohammad A Abdulhakeem, Mejdi Snoussi, Mohd Adnan Kausar
{"title":"Molecular identification, antibiotic susceptibility, and biofilm formation of airborne bacteria.","authors":"Malik Asif Hussain, Mubashir Zafar, Yusuf Saleem Khan, Kamaleldin B Said, Sadaf Anwar, Mohd Saeed, Mohammad A Abdulhakeem, Mejdi Snoussi, Mohd Adnan Kausar","doi":"10.1186/s13568-025-01861-1","DOIUrl":null,"url":null,"abstract":"<p><p>Pathogenic bacterial communities present in urban green spaces significantly affect human health, particularly for immunocompromised populations. The diverse range of pathogenic bacteria found in these areas poses substantial management challenges because of their high prevalence of antibiotic resistance, which can be life-threatening, particularly for immunocompromised individuals, including older adults and children. This study identified airborne bacterial species from 14 natural parks in the Hail region of the Kingdom of Saudi Arabia. Bacterial colonies isolated on blood agar plates were purified and characterised based on their morphological traits and their ability to secrete various virulence factors. A total of 28 distinct airborne bacterial species were isolated and purified. Antibiotic susceptibility tests revealed high resistance to fosfomycin (41.17%), ampicillin (17.64%), tetracycline (17.64%), and gentamicin (11.76%). Biofilm formation was evaluated qualitatively by slime production and quantitatively by crystal violet technique. The results revealed that 41.17% of the tested strains were non biofilm producers on polystyrene surfaces, 17.64% were weak biofilm formers, and 23.52% exhibited moderate biofilm formation. Notably, six strains exhibited strong biofilm-forming capabilities. Additionally, two bacteria from the Arthrobacter genus (A. crystallopoietes and A. saudimassiliensis) were identified. These findings provide valuable insights into the microbial composition of natural parks in the Hail region and highlight effective management strategies to mitigate health risks.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"74"},"PeriodicalIF":3.5000,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064487/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-025-01861-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Pathogenic bacterial communities present in urban green spaces significantly affect human health, particularly for immunocompromised populations. The diverse range of pathogenic bacteria found in these areas poses substantial management challenges because of their high prevalence of antibiotic resistance, which can be life-threatening, particularly for immunocompromised individuals, including older adults and children. This study identified airborne bacterial species from 14 natural parks in the Hail region of the Kingdom of Saudi Arabia. Bacterial colonies isolated on blood agar plates were purified and characterised based on their morphological traits and their ability to secrete various virulence factors. A total of 28 distinct airborne bacterial species were isolated and purified. Antibiotic susceptibility tests revealed high resistance to fosfomycin (41.17%), ampicillin (17.64%), tetracycline (17.64%), and gentamicin (11.76%). Biofilm formation was evaluated qualitatively by slime production and quantitatively by crystal violet technique. The results revealed that 41.17% of the tested strains were non biofilm producers on polystyrene surfaces, 17.64% were weak biofilm formers, and 23.52% exhibited moderate biofilm formation. Notably, six strains exhibited strong biofilm-forming capabilities. Additionally, two bacteria from the Arthrobacter genus (A. crystallopoietes and A. saudimassiliensis) were identified. These findings provide valuable insights into the microbial composition of natural parks in the Hail region and highlight effective management strategies to mitigate health risks.
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.