Intravenous Administration of Heterodimeric Phospholipase A2 HDP-1 Disturbs Cardiovascular System Functions and Biochemical Composition of Blood in Anesthetized Rats.

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-05-11 DOI:10.1134/S1607672925600137

A M Ismailova, M S Severyukhina, E R Shaykhutdinova, N A Perepechenova, V G Starkov, I A Dyachenko, V I Tsetlin, Yu N Utkin

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引用次数: 0

Abstract

Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two noncovalently bound subunits-HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have similar molecular masses and amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. After the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1, containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in blood biochemical parameters, indicating their damaging effect on cells and internal organs.

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静脉注射异二聚体磷脂酶A2 HDP-1对麻醉大鼠心血管系统功能和血液生化成分的影响

异二聚体磷脂酶A2 HDP-1由两个非共价结合的亚基hdp - 1p和HDP-1In组成，其中第一个具有溶脂活性，而第二个不具有。亚基具有相似的分子质量和氨基酸序列。麻醉大鼠给予HDP-1和HDP-1P可伴有持续的降压作用，其强度取决于剂量。在给予HDP-1P后，其效果比HDP-1的情况下发展得更慢，包含两个亚基，而单独的HDP-1In不影响血流动力学参数。通过心电图发现HDP-1和HDP-1P影响大鼠心脏功能，引起心律失常，这可能表明它们能够影响心脏的神经支配。HDP-1及其亚基的使用也会导致血液生化参数的改变，表明它们对细胞和内脏的破坏作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.