Intravenous Administration of Heterodimeric Phospholipase A2 HDP-1 Disturbs Cardiovascular System Functions and Biochemical Composition of Blood in Anesthetized Rats.
A M Ismailova, M S Severyukhina, E R Shaykhutdinova, N A Perepechenova, V G Starkov, I A Dyachenko, V I Tsetlin, Yu N Utkin
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引用次数: 0
Abstract
Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two noncovalently bound subunits-HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have similar molecular masses and amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. After the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1, containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in blood biochemical parameters, indicating their damaging effect on cells and internal organs.
期刊介绍:
Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.