Electrochemiluminescence lateral flow immunosensor using luminol-labeled silver nanoparticles for highly sensitive and quantitative detection of cardiac troponin I.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-15 DOI:10.1016/j.talanta.2025.128159

Jun Chen, Xuanxu Nan, Li Yang, Yue Cui

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引用次数: 0

Abstract

Cardiac troponin I (cTnI) is a highly specific biomarker of cardiomyocyte injury, released during cell disintegration and necrosis, and is the gold standard for diagnosing acute myocardial infarction (AMI). At the onset of AMI, cTnI appears in very low concentrations (pg/mL level), necessitating the development of highly sensitive and rapid detection sensors. In this study, an electrochemiluminescence lateral flow immunosensor (ECL-LFI) was designed using luminol-labeled silver nanoparticles (luminol@AgNPs) for the sensitive and quantitative detection of cTnI. A screen-printed electrode (SPE) was integrated beneath the nitrocellulose (NC) membrane with plastic plates of the same thickness applied on both sides of the SPE to ensure a smooth flow surface. Upon addition of cTnI and the luminol-H₂O₂ system, sandwich immune complexes formed by antibody-functionalized luminol@AgNPs on the strips generated electrochemiluminescent (ECL) signals. The ECL-LFI exhibited a broad linear detection range from 5 pg/mL to 100 ng/mL, with a detection limit as low as 1.6 pg/mL. Additionally, the results show excellent correlation with clinical tests, demonstrating that the ECL-LFI provides a promising point-of-care tool for the early diagnosis of AMI and other diseases.

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电化学发光侧流免疫传感器使用鲁米诺标记银纳米粒子高灵敏度和定量检测心脏肌钙蛋白I。

心肌肌钙蛋白I （cTnI）是一种高度特异性的心肌细胞损伤生物标志物，在细胞解体和坏死过程中释放，是诊断急性心肌梗死（AMI）的金标准。AMI发病时，cTnI的浓度非常低（pg/mL），需要开发高灵敏度和快速检测传感器。本研究设计了一种电化学发光侧流免疫传感器（ECL-LFI），使用鲁米诺标记的银纳米颗粒（luminol@AgNPs）进行cTnI的灵敏定量检测。将丝网印刷电极（SPE）集成在硝化纤维素（NC）膜下，并在SPE两侧施加相同厚度的塑料板以确保其光滑的流动表面。在加入cTnI和鲁米诺- h2o2系统后，由抗体功能化luminol@AgNPs在条带上形成的三明治免疫复合物产生电化学发光（ECL）信号。ECL-LFI的线性检测范围为5 pg/mL ~ 100 ng/mL，检出限低至1.6 pg/mL。此外，结果显示与临床试验的良好相关性，表明ECL-LFI为AMI和其他疾病的早期诊断提供了一个有前途的即时护理工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.