Intravital Optical Bioimaging of Ovarian Cancer Using a Luminescent Cell Line.

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
E I Shramova, G M Proshkina, S M Deyev
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引用次数: 0

Abstract

Intravital bioimaging based on luminescence is an important method for the development and testing of antitumor drugs on model animals and is an essential part of preclinical studies. Bioimaging based on luminescent systems, compared with fluorescent bioimaging, provides a high signal-to-noise ratio, which justifies the development of cell lines that stably express luciferase genes for their subsequent use in model animals. In this work, we describe the creation of a stable cell line SKOV3.ip1-NanoLuc constitutively expressing the NanoLuc luciferase gene. The developed cell line was shown to be effective for intravital luminescence bioimaging of immunodeficient animals with deep-seated intraperitoneal tumors, which can be considered as a model of late-stage ovarian cancer.

利用发光细胞系对卵巢癌进行活体光学生物成像。
基于发光的活体生物成像是抗肿瘤药物在模型动物上开发和测试的重要方法,是临床前研究的重要组成部分。与荧光生物成像相比,基于发光系统的生物成像提供了高信噪比,这证明了能够稳定表达荧光素酶基因的细胞系的发展,以便随后在模型动物中使用。在这项工作中,我们描述了一个稳定的细胞系SKOV3的创建。ip1-NanoLuc组成性表达NanoLuc荧光素酶基因。所建立的细胞系对深部腹腔肿瘤免疫缺陷动物的活体发光生物成像有效,可作为晚期卵巢癌的一种模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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