Immunophenotypes of Systemic Lupus Erythematosus-Features of Clinical and Laboratory Disorders.

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
A S Avdeeva, A P Aleksankin, E V Chetina, Yu N Gorbunova, T V Popkova, G A Markova, T A Panafidina, E L Nasonov
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引用次数: 0

Abstract

Objective: to evaluate subpopulations of B lymphocytes and features of interferon (IFN) status in patients with systemic lupus erythematosus (SLE), to clarify the relationship of immunological parameters with clinical manifestations of the disease.

Materials and methods: : A total of 139 patients (123 women (88%) and 16 men (12%)) with a definite diagnosis of SLE were included in the analysis. The disease duration was 3.0 [0.3; 12.0] years, SLEDAI-2K 7 [4; 11] points, SDI 0 [0; 1] points. Immunophenotyping of peripheral blood lymphocytes, including determination of B cells, the general population of memory B cells, non-switched and switched memory B cells, naive, transient B cells, and plasmablasts was carried out using multicolor flow cytometry. IFN status was assessed by the expression of IFN-stimulated genes (MX1, RSAD2, and EPSTI1) using real-time polymerase chain reaction.

Results: . Two immunological "patterns" were identified-the prevailing immunological mechanism of the pathogenesis of the disease (SLE) with predominant activation of type I IFN and with predominant activation of the B-cell component of the immune system. The immunological phenotype with activation of type I IFN was associated with high immunological activity, predominant skin damage, and leukopenia, whereas the phenotype with predominant activation of the B-cell component was associated with damage to the kidneys and nervous system.

Conclusions: . The results of the work suggest a wide variety of immune mechanisms underlying the pathogenesis of SLE. It is possible to identify a number of leading molecular "patterns" of the pathogenesis of the disease, which must be taken into account to select an effective "targeted" drug.

系统性红斑狼疮的免疫表型-临床和实验室疾病的特征。
目的:评价系统性红斑狼疮(SLE)患者B淋巴细胞亚群及干扰素(IFN)状态特征,阐明免疫参数与疾病临床表现的关系。材料与方法:共纳入139例明确诊断为SLE的患者,其中女性123例(88%),男性16例(12%)。病程3.0 [0.3];12.0]年,SLEDAI-2K 7 [4];11]分,SDI 0 [0;1)点。采用多色流式细胞术对外周血淋巴细胞进行免疫分型,包括B细胞、一般记忆B细胞群、非开关和开关记忆B细胞、初始B细胞、瞬时B细胞和浆母细胞的测定。利用实时聚合酶链反应,通过IFN刺激基因(MX1、RSAD2和EPSTI1)的表达来评估IFN状态。结果:。确定了两种免疫“模式”——疾病(SLE)发病的主要免疫机制,主要是I型IFN的激活和免疫系统的b细胞成分的激活。I型IFN激活的免疫表型与高免疫活性、显性皮肤损伤和白细胞减少有关,而显性b细胞成分激活的表型与肾脏和神经系统损伤有关。结论:。研究结果表明SLE的发病机制存在多种免疫机制。有可能确定疾病发病机制的一些主要分子“模式”,必须考虑到选择有效的“靶向”药物。
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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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