Isolation of Fluconazole-Resistant South Asian Clade Candida auris in Beijing, China.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-04-22 DOI:10.4269/ajtmh.24-0748

Linlin Yan, Fan Zhao, Ke Zhang, Pengfei Wang, Shoukui Hu

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Abstract

Since first isolate of Candida (C.) auris was recognized in 2018 in China, over 60 cases of C. auris colonization or infection have been reported up to 2022, with at least two distinct genetic clade. Most isolates from South Asian clade and South African clade were sensitive and resistant to fluconazole, respectively. Here, we report an isolate of fluconazole-resistant South Asian clade C. auris (PUSH159) from blood. The efflux pump genes MDR1, CDR1, CDR4, SNQ2, and ARB1, and ergosterol biosynthesis gene ERG11 were detected in strain PUSH159. Single nucleotide polymorphism (SNP) analysis revealed that strain PUSH159 carried multiple mutations in drug resistance-associated genes, including a hot-spot mutation (Y132F) in ERG11, missense mutation of E709D in CDR1, and missense mutations of K52N and E464K in SNQ2. Our findings support the genetic diversity and differences in antifungal susceptibility within the South Asian clade, and provide early warning for the emergence of fluconazole-resistant South Asian clade isolates in China.

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北京地区耐氟康唑南亚分支耳念珠菌的分离。

自2018年中国首次发现耳念珠菌（C.）分离株以来，截至2022年，已报告了60多例耳念珠菌定植或感染病例，至少有两个不同的遗传进化支。大多数来自南亚分支和南非分支的分离株分别对氟康唑敏感和耐药。在这里，我们报告了从血液中分离出的耐氟康唑的南亚分支C. auris （PUSH159）。在菌株PUSH159中检测到外排泵基因MDR1、CDR1、CDR4、SNQ2、ARB1和麦角甾醇生物合成基因ERG11。单核苷酸多态性（SNP）分析显示，菌株PUSH159携带多个耐药相关基因突变，包括ERG11热点突变（Y132F）、CDR1 E709D错义突变、SNQ2 K52N和E464K错义突变。我们的研究结果支持南亚分支中抗真菌敏感性的遗传多样性和差异，并为中国出现耐氟康唑南亚分支分离株提供早期预警。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.20

自引率

3.00%

发文量

508

审稿时长

3 months

期刊介绍： The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine. The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development. The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal. Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries