Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-05-05 DOI:10.1007/s10529-025-03594-4

Francisco de la Fuente-Kratzborn, Qin Fan, Peter Neubauer, Matthias Gimpel

引用次数: 0

Abstract

Objectives: This study aims to assess whether a fed-batch-based auto-induction method can enhance active hydrogenase production, encompassing cofactor formation and space-time yield.

Results: The recombinant Escherichia coli strain BQF8RH8, possessing plasmids for assembly and proper maturation of Cupriavidus necator regulatory hydrogenase (RH), was cultivated in the fed-batch like EnPresso B medium with an autoinduction lactose-based system. In contrast to classical IPTG induction previously performed, we obtained active RH demonstrating the feasibility of the process for active hydrogenase production.

Conclusion: Our results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.

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乳糖自诱导在大肠杆菌中异种生产活性[NiFe]氢化酶中的应用。

目的：本研究旨在评估基于补料批量的自动诱导方法是否可以提高活性氢化酶的产量，包括辅因子的形成和时空产率。结果：重组大肠杆菌BQF8RH8在EnPresso B培养基中以自诱导乳糖为基础培养，具有组装和成熟铜绿调节氢化酶（RH）的质粒。与之前进行的经典IPTG诱导相比，我们获得了活性RH，证明了活性加氢酶生产过程的可行性。结论：我们的研究结果证实了先前开发的具有复杂成熟过程的功能性氢化酶的自动诱导策略的可行性。这大大加快了过程，提高了氢化酶的生产力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.