Complementary dual-modal immunosensing strategy for accurate detecting zearalenone based on bimetallic NiFe-Prussian blue analogue.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-10-01 Epub Date: 2025-04-19 DOI:10.1016/j.talanta.2025.128165

Bin Hu, Xiaowu Tang, Yinpeng Zhang, Yiming Ruan, Xiaohong Shang, Fenghe Duan, Shuai Zhang, Zhihong Zhang

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引用次数: 0

Abstract

In this work, a complementary dual-modal immunosensing strategy was fabricated for the sensitive and selective detection of zearalenone (ZEN) by integrating immunochromatographic and electrochemical biosensing methods. For this, bimetallic NiFe Prussian blue analogue (NiFe-PBA) was simultaneously employed as a matrix for labelling ZEN-targeted antibody and the sensitive layer for anchoring antibody to construct an efficient immunochromatographic immunosensor (ICI) and electrochemical immunosensor (ECI), respectively. Due to porous network, good functionality, high bioaffinity, and rich metal redox, NiFe-PBA-based complementary dual-modal immunosensor exhibited low limit of detection, high selectivity and stability, and accepted practicality. Particularly, the developed ECI illustrated fast response, portability, and potentially commercial application. Coupling the ICI and ECI sensing strategy can markedly enhance the accuracy and sensitive detection of ZEN in complicated environments. This dual-modal ICI-ECI immunosensing strategy exhibits significant potential for the precise detection of mycotoxin in various agricultural products and improve the quality control for foods.

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基于双金属镍铁-普鲁士蓝类似物的玉米赤霉烯酮精确检测互补双峰免疫传感策略。

本研究结合免疫层析和电化学生物传感技术，构建了一种互补的双模免疫传感策略，用于玉米霉烯酮（ZEN）的敏感和选择性检测。为此，采用双金属NiFe普鲁士蓝类似物（NiFe- pba）同时作为标记zen靶向抗体的基质和锚定抗体的敏感层，分别构建高效的免疫层析免疫传感器（ICI）和电化学免疫传感器（ECI）。基于nfe - pba的互补双模免疫传感器具有多孔网络、良好的功能、高的生物亲和性和丰富的金属氧化还原性，具有低检测限、高选择性和稳定性，具有广泛的实用性。特别是，开发的ECI显示了快速响应、可移植性和潜在的商业应用。耦合ICI和ECI传感策略可以显著提高复杂环境下ZEN的检测精度和灵敏度。这种双模态ci - eci免疫感应策略在各种农产品中霉菌毒素的精确检测和提高食品质量控制方面具有重要的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.