Jihye Lee, Byeolnim Oh, Jaewon Park, Jee Young Kim, Jino Son, Hyun Soo Kim, Yoon-E Choi
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引用次数: 0
Abstract
To detect microcystin-LR (MC-LR), a potent cyanotoxin harmful to human health, fast and precise monitoring tools are essential, particularly for on-site applications. In this study, we developed a novel on-site microfluidic aptasensor system that utilizes a fluorescence-tagged aptamer for the rapid and selective detection of MC-LR. By employing a target non-immobilized aptamer selection technique, high-affinity DNA aptamers for MC-LR were identified, and then further optimized through sequence truncation to enhance detection efficiency. The developed aptamer was designed such that its fluorescence is quenched in the absence of MC-LR, but recovered in its presence, enabling a clear signal detection that correlates with a toxin concentration. The developed system achieved a detection limit of 1.9 ppb, significantly lower than the safety threshold suggested by world health organization (WHO) for recreational waters, demonstrating sufficient sensitivity for reliable on-site monitoring. In addition, the microfluidic aptasensor system demonstrated high specificity, exhibiting the strongest response to MC-LR compared to other cyanotoxins. This system is the first portable MC-LR detection tool that allows for on-site environmental monitoring with the advantages of its fast response time and ease of operation. Combining a low detection limit with high accuracy, the microfluidic aptasensor system presents a promising alternative to conventional laboratory-based methods, providing a practical and reliable solution for water quality assessment.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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