Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids.

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-05-06 DOI:10.3390/ani15091338
Balázs Libisch, Zsuzsanna J Sándor, Tibor Keresztény, Chioma Lilian Ozoaduche, Péter P Papp, Katalin Posta, Janka Biró, Viktor Stojkov, Vojislav Banjac, Nóra Adányi, Mária Berki, Éva Lengyel-Kónya, Rita Tömösközi-Farkas, Ferenc Olasz
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Abstract

The impact of short-term feeding of three distinct diets containing insect meals on the intestinal microbiota of African catfish hybrid (Clarias gariepinus × Heterobranchus longifilis) juveniles was examined. The animals received experimental diets containing 30% insect meals derived from black soldier-fly larvae (BSL) (Hermetia illucens), yellow mealworm (Tenebrio molitor) or blue bottle-fly larvae (Calliphora vicina) for 18 days. The relative abundance of the Bacillaceae, the Planococcaceae and other bacteria significantly increased (p < 0.05) in the intestinal microbiota of the BSL group and also in the pooled group of the three catfish groups that received insect meals. Several strains of the Bacillales cultured from BSL feed had higher (p < 0.05) abundance in the intestinal microbiota of the BSL group compared to those of the control group. Among these Bacillales strains, a single fosB antibiotic resistance gene was identified. In the gut resistomes of both the BSL and the control catfish groups, the tetA(P), tetB(P) and lnu(C) antibiotic-resistance determinants were detected, while fosB was detected only in the BSL group. Overall, the study showed that a short-term shift to diets containing insect meals can induce significant (q < 0.05) changes in the gut microbiota of the African catfish without the development of reduced α-diversity and without the overgrowth of bacteria pathogenic to fish.

短期投喂昆虫粉对杂交非洲鲶鱼肠道菌群的影响。
研究了短期饲养3种不同昆虫饲料对杂交非洲鲶鱼(Clarias gariepinus × Heterobranchus long - filis)幼鱼肠道微生物群的影响。实验饲粮中含有30%的黑虻幼虫(Hermetia illucens)、黄粉虫(Tenebrio molitor)或蓝瓶蝇幼虫(Calliphora vicina)虫粕,饲喂18 d。食用昆虫饲料的3组鲶鱼肠道菌群中杆菌科、Planococcaceae等细菌的相对丰度均显著增加(p < 0.05), BSL组和混合组也显著增加(p < 0.05)。用BSL饲料培养的部分芽孢杆菌在BSL组肠道菌群中的丰度高于对照组(p < 0.05)。在这些芽胞杆菌菌株中,鉴定出一个单一的fosB耐药基因。在BSL组和对照组的肠道抗性组中均检测到tetA(P)、tetB(P)和lnu(C)耐药决定因素,而fosB仅在BSL组中检测到。综上所示,短期内改用昆虫饲料可诱导非洲鲶鱼肠道菌群发生显著(q < 0.05)变化,但不会导致α-多样性降低,也不会导致致病性细菌过度生长。
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来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
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