Development of a Method for Visualizing and Quantifying Thrombus Formation in Extracorporeal Membrane Oxygenators.

IF 2.3 4区医学 Q3 BIOPHYSICS

Cellular and molecular bioengineering Pub Date : 2025-04-12 eCollection Date: 2025-04-01 DOI:10.1007/s12195-025-00847-0

Jenny S H Wang, Amelia A Rodolf, Caleb H Moon, Ari Lauthner, Helen H Vu, Sandra Rugonyi, Anna J Hansen, Heather M Mayes, Bishoy Zakhary, David Zonies, Ran Ran, Akram Khan, Denis Wirtz, Ashley L Kiemen, Owen J T McCarty, Joseph J Shatzel

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引用次数: 0

Abstract

Purpose: Extracorporeal membrane oxygenation (ECMO) is a life-saving critical care technology that presents significant risks of medical device-associated thrombosis. We developed a complete method for collecting membrane oxygenators (membrane lung) from patients receiving ECMO treatment and quantitatively analyzing the distribution of thrombus formation within the membrane.

Methods: We collected used membrane oxygenators from patients for processing and imaging with microcomputed tomography (microCT). We reconstructed the microCT data and performed image segmentation to identify regions of thrombus formation within these oxygenators. We performed density mapping to quantify thrombus volume across different regions of each oxygenator and within multiple oxygenator models.

Results: Our method yields two-dimensional and three-dimensional visualization and quantification of thrombus deposition in ECMO. Analysis of the spatial distribution of platelet deposition, red blood cell entrapment, and fibrin formation within the fouled device provides insights into the structural patterns of oxygenator thrombosis.

Conclusions: This method can enable quantification of oxygenator thrombosis which can be used for evaluating the effect of new biomaterial or pharmacological approaches for mitigating vascular device-associated thrombosis during ECMO.

Supplementary information: The online version contains supplementary material available at 10.1007/s12195-025-00847-0.

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体外膜氧合器血栓形成可视化和定量方法的发展。

目的：体外膜氧合（ECMO）是一种挽救生命的危重监护技术，存在医疗器械相关血栓形成的重大风险。我们开发了一种完整的方法，从接受ECMO治疗的患者收集膜氧合器（膜肺），并定量分析膜内血栓形成的分布。方法：收集患者使用过的膜氧合器，进行显微计算机断层扫描（microCT）处理和成像。我们重建了微ct数据，并进行了图像分割，以确定这些氧合器内血栓形成的区域。我们进行了密度测绘，以量化每个氧合器不同区域和多个氧合器模型内的血栓体积。结果：本方法可实现ECMO内血栓沉积的二维和三维可视化和量化。血小板沉积的空间分布分析，红细胞包裹，纤维蛋白形成在污染的设备提供洞察氧合器血栓形成的结构模式。结论：该方法可以定量氧合器血栓形成，可用于评价新的生物材料或药物治疗方法在ECMO中减轻血管装置相关血栓形成的效果。补充信息：在线版本包含补充资料，可在10.1007/s12195-025-00847-0获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.