Fluorescence-Based Detection of Methionine for Noninvasive Cancer Diagnosis Using Cu-Sn Bimetallic Nanoclusters.

IF 3.6 4区医学 Q2 CHEMISTRY, MEDICINAL

ChemMedChem Pub Date : 2025-04-24 DOI:10.1002/cmdc.202500064

Geneva Indongo, Susan Varghese, Merin K Abraham, Greeshma Rajeevan, Arathy B Kala, Dheyaa Mohammed Dhahir, Sony George

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Abstract

Methionine is an essential sulfur-containing amino acid that plays a pivotal role in cancer biology due to its abnormal metabolism in malignant cells. Elevated methionine levels serve as potential biomarkers for various cancers, highlighting their diagnostic significance. This study presents a bimetallic sensing platform using L-cysteine-capped copper-tin nanoclusters (Cu-SnNCs) for the selective and sensitive detection of methionine. The Cu-SnNCs are synthesized via a one-pot hydrothermal process, exhibiting strong blue fluorescence, high stability, and significant optical properties. Fe³⁺ is employed as a quencher, leveraging its paramagnetic nature to suppress fluorescence, which is subsequently restored upon the addition of methionine. The sensing mechanism demonstrates a linear response over a methionine concentration range of 0.18-1.62 mm, with a detection limit of 1.69 μm. The probe's potential is further validated with a preliminary paper strip test for detecting methionine in biological samples. These findings underscore the utility of Cu-SnNCs as a noninvasive, economical diagnostic tool for cancer detection and screening.

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基于Cu-Sn双金属纳米团簇荧光检测蛋氨酸的非侵袭性癌症诊断。

蛋氨酸是一种必需的含硫氨基酸，由于其在恶性细胞中的代谢异常，在肿瘤生物学中起着举足轻重的作用。蛋氨酸水平升高可作为多种癌症的潜在生物标志物，突出其诊断意义。本研究提出了一种利用l -半胱氨酸覆盖铜锡纳米簇（cu - snnc）选择性灵敏检测蛋氨酸的双金属传感平台。采用一锅水热法合成Cu-SnNCs，具有强蓝色荧光、高稳定性和显著的光学性能。采用Fe3+作为猝灭剂，利用其顺磁性抑制荧光，随后在加入蛋氨酸后恢复荧光。该传感机制在0.18 ~ 1.62 mM的蛋氨酸浓度范围内呈线性响应，检测限为1.69µM。该探针的潜力通过初步试纸条试验进一步验证，用于检测生物样品中的蛋氨酸。这些发现强调了cu - snnc作为一种无创、经济的癌症检测和筛查诊断工具的实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ChemMedChem 医学-药学

CiteScore

6.70

自引率

2.90%

发文量

280

审稿时长

1 months

期刊介绍： Quality research. Outstanding publications. With an impact factor of 3.124 (2019), ChemMedChem is a top journal for research at the interface of chemistry, biology and medicine. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. ChemMedChem publishes primary as well as critical secondary and tertiary information from authors across and for the world. Its mission is to integrate the wide and flourishing field of medicinal and pharmaceutical sciences, ranging from drug design and discovery to drug development and delivery, from molecular modeling to combinatorial chemistry, from target validation to lead generation and ADMET studies. ChemMedChem typically covers topics on small molecules, therapeutic macromolecules, peptides, peptidomimetics, and aptamers, protein-drug conjugates, nucleic acid therapies, and beginning 2017, nanomedicine, particularly 1) targeted nanodelivery, 2) theranostic nanoparticles, and 3) nanodrugs. Contents ChemMedChem publishes an attractive mixture of: Full Papers and Communications Reviews and Minireviews Patent Reviews Highlights and Concepts Book and Multimedia Reviews.