Time- and season-dependent changes in the steroidogenic markers in female tree sparrow (Passer montanus).

Abstract

Seasonal breeders display elevated sex steroid hormone production during reproductive seasons, resulting in significant physiological and structural alterations. One such seasonal breeder adapted to the changing environment is a Tree sparrow (Passer montanus). The study aims to investigate 24-h rhythmicity and annual variations in the expression of steroidogenic gene markers of adult female tree sparrows. Two experiments were conducted; in experiment one, birds (n = 5 birds/time points) were sampled at six time points, i.e., ZT1, ZT5, ZT9, ZT13, ZT17, and ZT21 (ZT = Zeitgeber time, ZT0 = sunrise time) during the reproductive stage; subsequently, hypothalamus and ovary were harvested for gene expression analysis. In experiment two, birds (n = 5/month) were sampled at mid-day every month for a year. Feather molt, follicular diameter, body mass, and bill coloration were recorded. The hypothalamus and ovary were harvested for gene expression studies. Blood plasma cholesterol and progesterone were also measured. The study indicates a larger follicular size during May and June. Whereas, maximum molt was observed during the post-reproductive phase. Cholesterol levels were highest prior breeding phase and higher progesterone levels paralleled larger follicular size. While higher levels of GnIh (gonadotropin-inhibitory hormone) and Dio3 (type 3 deiodinase) were observed during the non-breeding phase, elevated expression of Tshβ (thyroid stimulating hormone subunit beta), Dio2 (type 2 deiodinase), and GnRh (gonadotropin-releasing hormone) was noted during the reproductive period. The study also reveals 24-h rhythmicity in selected steroidogenic markers (StAR, Nr4a1, Er, Scp2, Cyp17a1, Foxl2, Cyp11a1, Hsd11b2, Cyp11b, Cyp19a1, and Vdac1) and seasonal variations directly influence steroidogenesis, which connects with the annual reproductive cycle.