Effects of Thymus daenensis Essential Oil-loaded chitosan Nanoparticles on BCR1 Gene Expression in Candida Parapsilosis.

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.32592/ARI.2024.79.5.973
Z Hadi, A Ferdousi, M Paknejadi
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Abstract

Candida parapsilosis is a non-albicans species with a high prevalence and potential for nosocomial infections. The BCR1 gene plays a critical role in regulating virulence factors in this species. This study aimed to evaluate the effects of Thymus daenensis essential oil encapsulated in chitosan nanoparticles (TDNs) on the expression of the BCR1 gene in C. parapsilosis isolates from animal and human sources. Sixty C. parapsilosis isolates (30 from human and 30 from veterinary sources) were screened for the presence of the BCR1 gene via PCR. The TDNs were synthesized and characterized using various techniques. The isolates carrying the BCR1 gene were treated with TDNs to determine the minimum inhibitory concentration (MIC). The expression of the BCR1 gene after treatment with sub-MIC concentrations of TDNs was measured by real-time PCR and compared with the control group. The results were statistically analyzed. Five out of 60 isolates (8.33%) tested positive for the BCR1 gene. The physical properties of TDNs showed that they had a spherical shape, an average size of 92.3 nm, a polydispersity index of 0.129±0.03, a zeta potential of +48.3 mV, and an encapsulation efficiency of 88.6 ± 0.2%. The MIC range for TDNs in these isolates was 0.032-1 μg/ml. Treatment with TDNs significantly reduced the expression of the BCR1 gene in all five isolates compared with the control group (p=0.012). TDN has substantial potential for inhibiting the expression of the BCR1 gene, associated with virulence in C. parapsilosis. This may enhance the antifungal activity of TDN and reduce the risk of nosocomial infections caused by this species.

载胸腺精油壳聚糖纳米颗粒对假丝酵母菌BCR1基因表达的影响
假丝酵母菌是一种非白色念珠菌,具有很高的患病率和院内感染的潜力。BCR1基因在该物种的毒力因子调控中起关键作用。本研究旨在研究壳聚糖纳米颗粒包封胸腺精油(TDNs)对人源和动物源的巨孢子虫BCR1基因表达的影响。采用PCR方法对60株假丝裂菌(30株来自人源,30株来自兽源)进行了BCR1基因的筛选。利用各种技术合成并表征了tdn。用TDNs处理携带BCR1基因的分离株,测定最低抑菌浓度(MIC)。采用实时荧光定量PCR检测亚mic浓度tdn治疗后BCR1基因的表达,并与对照组进行比较。对结果进行统计学分析。60株分离株中5株(8.33%)BCR1基因检测阳性。物理性质表明,tdn呈球形,平均尺寸为92.3 nm,多分散性指数为0.129±0.03,zeta电位为+48.3 mV,包封效率为88.6±0.2%。tdn的MIC范围为0.032 ~ 1 μg/ml。与对照组相比,TDNs治疗显著降低了所有5个分离株BCR1基因的表达(p=0.012)。TDN具有抑制BCR1基因表达的巨大潜力,这与巨噬菌的毒力有关。这可能增强了TDN的抗真菌活性,降低了该物种引起的医院感染的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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