An efficient method for the selective isolation of feline herpesvirus 1(FHV-1) in feline calicivirus (FCV) coinfected specimens.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-05-07 DOI:10.1186/s12917-025-04786-w

Huanqin Zheng, Hong Yue, Baoyan Wang, Xin Yu, Yang Liu, Jiayu Yu, Jianlong Zhang, Kexue Han, Yinuo Han, Hanfeng Su, Hongwei Zhu, Xingxiao Zhang

{"title":"An efficient method for the selective isolation of feline herpesvirus 1(FHV-1) in feline calicivirus (FCV) coinfected specimens.","authors":"Huanqin Zheng, Hong Yue, Baoyan Wang, Xin Yu, Yang Liu, Jiayu Yu, Jianlong Zhang, Kexue Han, Yinuo Han, Hanfeng Su, Hongwei Zhu, Xingxiao Zhang","doi":"10.1186/s12917-025-04786-w","DOIUrl":null,"url":null,"abstract":"Background: Feline herpesvirus 1 (FHV-1) and feline calicivirus (FCV) are the most common viral pathogens of feline respiratory disease and are highly prevalent in cats worldwide. Coinfection with these viruses is frequent in cats with feline respiratory disease complex (FRDC). It is difficult to isolate pure FHV-1 by conventional laboratory cell culture methods from specimens with FRDC, which brings great trouble to the epidemiological investigation of FHV-1.Methods: FCV polyclonal antibodies were obtained by immunizing rabbits, and the coinfected specimens were neutralized with FCV polyclonal antibodies. Then, virus isolation was performed. After several rounds of neutralization, FHV-1 was finally obtained.Results: The FCV polyclonal antibody was successfully obtained with neutralizing activities of 1:128, 1:537, and 1:91. After virus neutralization, the FHV-1 was successfully isolated from the coinfected cell culture suspension and confirmed by immunofluorescence and QRT-PCR.Conclusion: In this study, all FHV-1 present in the coinfection samples were isolated, without any cross-contamination. This method is also theoretically suitable for the isolation and purification of other FCV coinfections or contaminating disease substances.","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"321"},"PeriodicalIF":2.3000,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057004/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12917-025-04786-w","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Feline herpesvirus 1 (FHV-1) and feline calicivirus (FCV) are the most common viral pathogens of feline respiratory disease and are highly prevalent in cats worldwide. Coinfection with these viruses is frequent in cats with feline respiratory disease complex (FRDC). It is difficult to isolate pure FHV-1 by conventional laboratory cell culture methods from specimens with FRDC, which brings great trouble to the epidemiological investigation of FHV-1.

Methods: FCV polyclonal antibodies were obtained by immunizing rabbits, and the coinfected specimens were neutralized with FCV polyclonal antibodies. Then, virus isolation was performed. After several rounds of neutralization, FHV-1 was finally obtained.

Results: The FCV polyclonal antibody was successfully obtained with neutralizing activities of 1:128, 1:537, and 1:91. After virus neutralization, the FHV-1 was successfully isolated from the coinfected cell culture suspension and confirmed by immunofluorescence and QRT-PCR.

Conclusion: In this study, all FHV-1 present in the coinfection samples were isolated, without any cross-contamination. This method is also theoretically suitable for the isolation and purification of other FCV coinfections or contaminating disease substances.

查看原文本刊更多论文

一种从猫杯状病毒（FCV）共感染标本中选择性分离猫疱疹病毒1型（FHV-1）的方法。

背景：猫疱疹病毒1型（FHV-1）和猫杯状病毒（FCV）是猫科呼吸道疾病最常见的病毒病原体，在全世界的猫中高度流行。同时感染这些病毒在患有猫呼吸道疾病（FRDC）的猫中很常见。传统的实验室细胞培养方法很难从FRDC标本中分离到纯FHV-1，这给FHV-1的流行病学调查带来了很大的麻烦。方法：通过免疫家兔获得FCV多克隆抗体，并用FCV多克隆抗体中和共感染标本。然后进行病毒分离。经过几轮中和，最终获得FHV-1。结果：成功获得FCV多克隆抗体，中和活性分别为1:128、1:537和1:91。病毒中和后，成功地从共感染细胞培养悬液中分离出FHV-1，并通过免疫荧光和QRT-PCR进行证实。结论：本研究共感染样本中FHV-1均被分离，无交叉污染。该方法理论上也适用于其他FCV共感染或污染疾病物质的分离纯化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.