Probiotic reduces vaginal HPV abundance, improves immunity and quality of life in HPV-positive women: a randomised, placebo-controlled and double-blind study.

IF 3 4区 医学 Q2 MICROBIOLOGY
P Xu, U Mageswary, A A Nisaa, S D Balasubramaniam, S B Samsudin, N I B M Rusdi, A R A Jerip, C E Oon, M H A Bakar, D Rajendran, J J Tan, F F Roslan, S Sreenivasan, V Balakrishnan, S B Sany, C S Tan, M T Liong
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引用次数: 0

Abstract

The Human Papillomavirus (HPV) is one of the main causes of cervical cancer in women, while there are currently no treatment nor intervention to reduce the concentration of cervical HPV. We thus aimed to investigate the effects of a probiotic Lactiplantibacillus plantarum Probio87 (orally administered at 9 log CFU/day) or placebo for 12-weeks, on reducing the abundance of vaginal HPV in HPV-positive women. A parallel, randomised, double-blind and placebo-controlled study was performed where women were randomised to either the probiotic (n = 44, mean age 41.70 ± 1.06 years) or placebo (n = 45, mean age 41.13 ± 1.20 years). After 12 weeks, the probiotic group showed reduced vaginal HPV abundance ( P = 0.001) and Nugent scores ( P < 0.001) as compared to the placebo. VAS and VuAS questionnaires showed that the probiotic group had improved vulvar dryness ( P = 0.023), soreness ( P = 0.049), social interactions, daily activities ( P < 0.05), and sexual activity ( P = 0.022) compared to the placebo group. Blood gene expressions showed that the placebo group had higher upregulation of pro-inflammatory cytokines (IL-1β, P = 0.006; IFN-γ, P = 0.028) and T-cell markers (CD44, P = 0.008; CXCR5, P = 0.040; CD4, P = 0.016) compared to the placebo group, indicating increased inflammation. Neurotrophic factors BDNF and CREB were upregulated in the placebo group ( P < 0.05), with higher IDO ( P = 0.001) and TDO ( P = 0.036) expressions compared to the probiotic group, suggesting increased kynurenine pathway activity and stress. Overall, probiotic supplementation appeared to reduce the abundance of vaginal HPV, possibly by lowering inflammation and enhancing immunity while mitigating the negative impacts of HPV infection on quality of life in HPV-positive women. Clinical trial registration: ClinicalTrials.gov (NCT05316064).

益生菌减少阴道HPV丰度,提高HPV阳性女性的免疫力和生活质量:一项随机、安慰剂对照和双盲研究。
人乳头瘤病毒(HPV)是导致妇女宫颈癌的主要原因之一,而目前没有治疗或干预措施来降低宫颈HPV的浓度。因此,我们的目的是研究益生菌植物乳杆菌Probio87(口服剂量为9 log CFU/天)或安慰剂对降低HPV阳性妇女阴道HPV丰度的影响。在一项平行、随机、双盲和安慰剂对照研究中,女性被随机分为益生菌组(n = 44,平均年龄41.70±1.06岁)和安慰剂组(n = 45,平均年龄41.13±1.20岁)。12周后,与安慰剂组相比,益生菌组阴道HPV丰度(P = 0.001)和纽金特评分(P < 0.001)降低。VAS和VuAS问卷调查显示,与安慰剂组相比,益生菌组在外阴干燥(P = 0.023)、疼痛(P = 0.049)、社会交往、日常活动(P < 0.05)和性活动(P = 0.022)方面均有改善。血液基因表达显示,安慰剂组促炎细胞因子(IL-1β, P = 0.006;IFN-γ, P = 0.028)和t细胞标志物(CD44, P = 0.008;Cxcr5, p = 0.040;CD4, P = 0.016)与安慰剂组相比,表明炎症增加。与益生菌组相比,安慰剂组神经营养因子BDNF和CREB表达上调(P < 0.05), IDO (P = 0.001)和TDO (P = 0.036)表达升高,提示犬尿氨酸途径活性和应激增加。总的来说,补充益生菌似乎可以减少阴道HPV的丰度,可能是通过降低炎症和增强免疫力,同时减轻HPV感染对HPV阳性妇女生活质量的负面影响。临床试验注册:ClinicalTrials.gov (NCT05316064)。
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来源期刊
Beneficial microbes
Beneficial microbes MICROBIOLOGY-NUTRITION & DIETETICS
CiteScore
7.90
自引率
1.90%
发文量
53
审稿时长
>12 weeks
期刊介绍: Beneficial Microbes is a peer-reviewed scientific journal with a specific area of focus: the promotion of the science of microbes beneficial to the health and wellbeing of man and animal. The journal contains original research papers and critical reviews in all areas dealing with beneficial microbes in both the small and large intestine, together with opinions, a calendar of forthcoming beneficial microbes-related events and book reviews. The journal takes a multidisciplinary approach and focuses on a broad spectrum of issues, including safety aspects of pro- & prebiotics, regulatory aspects, mechanisms of action, health benefits for the host, optimal production processes, screening methods, (meta)genomics, proteomics and metabolomics, host and bacterial physiology, application, and role in health and disease in man and animal. Beneficial Microbes is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as those of policy makers and regulators. The journal will have five major sections: * Food, nutrition and health * Animal nutrition * Processing and application * Regulatory & safety aspects * Medical & health applications In these sections, topics dealt with by Beneficial Microbes include: * Worldwide safety and regulatory issues * Human and animal nutrition and health effects * Latest discoveries in mechanistic studies and screening methods to unravel mode of action * Host physiology related to allergy, inflammation, obesity, etc. * Trends in application of (meta)genomics, proteomics and metabolomics * New developments in how processing optimizes pro- & prebiotics for application * Bacterial physiology related to health benefits
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