Unlocking the Synergy: ABA Seed Priming Enhances Drought Tolerance in Seedlings of Sweet Sorghum Through ABA-IAA Crosstalk.

IF 6 1区生物学 Q1 PLANT SCIENCES

Plant, Cell & Environment Pub Date : 2025-04-23 DOI:10.1111/pce.15575

Yao Luhua, Ni Yu, Chen Chunjie, Xiong Wangdan, Gan Qiaoqiao, Jia Xinfeng, Jin Shurong, Yang Jianfeng, Guo Yanjun

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引用次数: 0

Abstract

Abscisic acid (ABA) seed priming impacts plant growth and stress resistance, yet its precise physiological and molecular mechanisms remain elusive. This study explored the role of ABA-priming in enhancing drought acclimation in sweet sorghum (Sorghum bicolor Moench) using physiological assessments and comparative transcriptomics. Under drought stress, ABA-primed seedlings exhibited increased plant height, larger leaves, and higher leaf water content compared to non-primed plants. While drought negatively affected photosynthesis through the regulation of photosystem I and II, ABA-priming improved photosynthesis and WUE by involving in differential expression of photosystem II genes. ABA-priming promoted the accumulation of cuticular wax and cutin, effectively reducing leaf water loss. Drought triggered endogenous ABA production via ABA inactivation genes (UGT, BGLU), while ABA-priming activated auxin (IAA) biosynthesis via YUCCA, enhancing auxin-mediated responses and gibberellic acid (GA) signalling. The synergistic action of ABA and IAA culminated in enhanced drought tolerance. Additionally, ABA-priming and drought stress regulated NAC transcription factors, with SbNAC21-1 emerging as a pivotal transcriptional activator intricately linked to auxin signalling. Overexpression of SbNAC21-1 in Arabidopsis effectively enhanced drought tolerance. These findings offer valuable insights into the intricate mechanisms underpinning the beneficial effects of ABA-priming, ultimately enhancing plant adaptability to environmental stressors.

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ABA种子激发通过ABA- iaa串扰提高甜高粱幼苗抗旱性

ABA对植物生长和抗逆性有显著影响，但其具体的生理和分子机制尚不清楚。本研究利用生理评估和比较转录组学方法探讨了aba启动在甜高粱（sorghum bicolor Moench）干旱驯化中的作用。干旱胁迫下，aba处理的幼苗比未处理的幼苗株高、叶片大、叶片含水量高。干旱通过调控光系统I和II对光合作用产生负面影响，而aba引发通过参与光系统II基因的差异表达提高光合作用和水分利用效率。灌浆aba能促进角质层蜡质和角质层的积累，有效减少叶片水分流失。干旱通过ABA失活基因（UGT， BGLU）触发内源ABA的产生，而ABA启动通过YUCCA激活生长素（IAA）的生物合成，增强生长素介导的反应和赤霉素（GA）信号传导。ABA和IAA的协同作用最终增强了抗旱性。此外，aba启动和干旱胁迫调节NAC转录因子，其中SbNAC21-1成为与生长素信号传导复杂相关的关键转录激活因子。SbNAC21-1在拟南芥中过表达可有效增强耐旱性。这些发现为aba启动的有益作用的复杂机制提供了有价值的见解，最终增强了植物对环境胁迫的适应性。

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来源期刊

Plant, Cell & Environment 生物-植物科学

CiteScore

13.30

自引率

4.10%

发文量

253

审稿时长

1.8 months

期刊介绍： Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.