Mussel-Inspired MXene/Antimicrobial Peptide-Integrated Photosensitive Poly(vinyl alcohol)-Based Hydrogel with Antibacterial, Anti-Inflammatory, and Electroactive Properties for Accelerated Wound Healing.

Abstract

Backgrounds: The buildup of reactive oxygen species (ROS) in infected wounds triggers an excessive inflammatory response, while the overuse of antibiotics has contributed to increased bacterial resistance. Therefore, developing wound dressings that effectively eliminate ROS and inhibit bacterial growth is crucial. Methods: Inspired by mussel-derived proteins, we developed a polydopamine (PDA)-grafted MXene (PDA@MXene) and 3,4-dihydroxyphenylalanine-PonG1 (DOPA-PonG1)-modified photosensitive poly(vinyl alcohol) (PVA) hydrogel as a wound dressing. PDA@MXene was synthesized through dopamine self-polymerization on the MXene surface, while tyrosine hydroxylation was used to introduce DOPA into the antibacterial peptide ponericin G1 (PonG1). The hydrogel and its components were characterized, and their morphology was examined. The hydrogel's hemostatic ability, mechanical properties, and conductivity were evaluated. In vitro studies systematically evaluated antioxidative effects, antibacterial activity, biocompatibility, and expression of tissue regeneration-related factors. An infected full-thickness skin defect model was established in vivo, and different hydrogel treatments were applied. The wound-healing rate was then measured, followed by histological analysis using hematoxylin and eosin, Masson, Sirius Red, and immunofluorescence staining to investigate the healing mechanism. Results: The DOPA sequence enhanced PonG1 stability on the hydrogel surface, leading to sustained antibacterial ability. PDA@MXene significantly improved the hydrogel's conductivity and mechanical strength. Notably, the combined effects of DOPA-PonG1 and PDA@MXene contributed to enhanced antibacterial and ROS-scavenging properties. In vivo findings demonstrated that the DOPA-PonG1/PDA@MXene/PVA hydrogel accelerated infected wound healing by promoting angiogenesis and collagen deposition while reducing excessive inflammation. This study presents an innovative approach for treating infected wound defects and holds promise for clinical applications.