Margarita (Pearl) Extract Alleviates Melasma by Targeting CAMP-Responsive Element Binding Protein 1

IF 2.3 4区医学 Q2 DERMATOLOGY

Journal of Cosmetic Dermatology Pub Date : 2025-05-14 DOI:10.1111/jocd.70087

Liling Shen, Jia Yao

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引用次数: 0

Abstract

Purpose

Margarita (pearl) has detoxifying and skin barrier-repairing properties. The study was to evaluate the therapeutic effect of pearl on melasma and to preliminarily explore its possible mechanism of action.

Methods

The efficacy of pearl on patients with melasma was evaluated by the melasma area and severity index (MASI) and physician's global assessment (PGA) score. DNA sequencing was performed on pharyngeal swab samples from patients with melasma to obtain gene loci related to melasma. The active compounds and potential targets for pearl were retrieved from Integrated Traditional Chinese Medicine and HERB databases. The relevant genes of melasma were obtained from the GeneCards database and intersected with the targets of pearl to identify potential targets of pearl against melasma. The potential targets were mapped for the KEGG pathway in the KEGG Mapper and verified using human melanoma A375 cells that were exposed to ultraviolet irradiation.

Results

Pearl significantly improved the MASI and PGA scores of patients with melasma. DNA sequencing revealed that TYR and DCT were genes related to melasma. Calcium carbonate, iron, magnesium, manganese, silicon, strontium, and zinc were active compounds for pearl. CAMP-responsive element binding protein 1 (CREB1) was the target of pearl against melasma. Pearl regulated microphthalmia-associated transcription factors through CREB and affected melasma-related genes TYR and DCT, which in turn inhibit melanoma cell activity and intracellular tyrosinase activity.

Conclusion

Pearl can alleviate melasma by targeting the CREB1/MITF axis and then the melasma-related gene loci TYR and DCT.

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玛格丽塔（珍珠）提取物通过靶向camp响应元件结合蛋白1缓解黄褐斑

玛格丽塔（珍珠）具有排毒和修复皮肤屏障的特性。本研究旨在评价珍珠对黄褐斑的治疗作用，并初步探讨其可能的作用机制。方法采用黄褐斑面积及严重程度指数（MASI）和医师整体评分（PGA）评价珍珠治疗黄褐斑的疗效。对黄褐斑患者咽拭子样本进行DNA测序，获得与黄褐斑相关的基因位点。从中药中药综合数据库中检索珍珠的活性成分和潜在靶点。从GeneCards数据库中获取黄褐斑的相关基因，并与珍珠的靶点相交，确定珍珠抗黄褐斑的潜在靶点。在KEGG Mapper中绘制了KEGG通路的潜在靶点，并使用暴露于紫外线照射下的人类黑色素瘤A375细胞进行了验证。结果Pearl可显著改善黄褐斑患者的MASI和PGA评分。DNA测序结果显示，TYR和DCT是与黄褐斑相关的基因。碳酸钙、铁、镁、锰、硅、锶、锌是珍珠的活性化合物。CAMP-responsive element binding protein 1 （CREB1）是珍珠抗黄褐斑的靶点。Pearl通过CREB调控小眼相关转录因子，影响黄褐斑相关基因TYR和DCT，进而抑制黑色素瘤细胞活性和细胞内酪氨酸酶活性。结论珍珠可通过靶向CREB1/MITF轴，进而靶向黄褐斑相关基因位点TYR和DCT来缓解黄褐斑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cosmetic Dermatology DERMATOLOGY-

CiteScore

4.30

自引率

13.00%

发文量

818

审稿时长

>12 weeks

期刊介绍： The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques. The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.