Identification and Detection of Adulterations of Salmon Caviar by PCR, IR Spectrometry, and Digital Coloriometry

IF 1 4区 化学 Q4 CHEMISTRY, ANALYTICAL
V. G. Amelin, O. E. Emel’yanov, A. V. Tret’yakov, M. A. Gergel’, E. V. Zaitseva
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引用次数: 0

Abstract

A possibility of identifying and establishing adulterations of salmon caviar by a simple and an available method using PCR, Fourier transform IR spectrometry, digital colorimetry with the chemometric processing of the analysis results is shown. The PCR method is used to determine the species of salmon caviar. No salmon DNA in the studied caviar samples, as well as the presence of DNA of other fish, indicates the adulteration of the caviar products. Near- and mid-region Fourier transform infrared spectroscopy makes it possible to distinguish between the natural and imitation caviar after processing diffuse reflectance spectra by principal component analysis and hierarchical cluster analysis. The above methods are combined with a simpler and a cheaper colorimetric analysis method. Hand-made devices with LEDs emitting in the UV and IR regions are used. The analytical signal is recorded using smartphones with specialized applications. Chemometric processing of the spectral characteristics of the samples makes it possible to distinguish natural caviar from imitation and structured caviar: in the principal component analysis and hierarchical cluster analysis, points of the analyzed samples are located in different quadrants and clusters.

用PCR、红外光谱和数字比色法鉴别和检测鲑鱼鱼子酱的掺假
利用PCR、傅里叶变换红外光谱法、数字比色法对分析结果进行化学处理,证明了一种简单有效的方法可以鉴别和确定鲑鱼鱼子酱的掺假。采用PCR方法对三文鱼鱼子酱的种类进行了鉴定。鱼子酱样品中没有鲑鱼DNA,同时也没有其他鱼类的DNA,这表明鱼子酱产品存在掺假。近、中区域傅里叶变换红外光谱在对鱼子酱漫反射光谱进行主成分分析和层次聚类分析后,可以区分天然鱼子酱和仿鱼子酱。上述方法与一种更简单、更便宜的比色分析法相结合。手工制作的led设备在紫外和红外区域发射。分析信号是用带有专门应用程序的智能手机记录的。对样品的光谱特征进行化学计量学处理,可以区分天然鱼子酱、仿鱼子酱和结构化鱼子酱:在主成分分析和层次聚类分析中,分析样品的点位于不同的象限和聚类中。
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来源期刊
Journal of Analytical Chemistry
Journal of Analytical Chemistry 化学-分析化学
CiteScore
2.10
自引率
9.10%
发文量
146
审稿时长
13 months
期刊介绍: The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.
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