Effect of temperature and time on RNA detection by RT-qPCR in rodent tissue and blood samples stored in MagMAX™ Lysis/Binding Solution Concentrate: Considerations for RNA detection in specimens stored under suboptimal conditions

Abstract

For detection of viral RNA in blood or tissue, samples are often collected into lysis buffers prior to downstream molecular analysis. Immediate sample processing and cold storage are not always possible during large-scale or field studies, or in facilities lacking a stable electrical supply. Additionally, samples may need to be transported significant distances before processing. Here, using peptidylprolyl isomerase A (Ppia), a stably expressed gene in rodent tissues, we investigate the long-term stability and detection of RNA in guinea pig tissues stored for up to 52 weeks and in hamster blood stored for up to 12 weeks in MagMAX Lysis/Binding Solution Concentrate at −80°C, 4°C, 21°C, and 32°C.