Alternaria alstroemeriae F1 inhibits aspergillus flavus growth and weakens aflatoxins biosynthesis

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY

Fungal Genetics and Biology Pub Date : 2025-04-25 DOI:10.1016/j.fgb.2025.103989

Yingying Ye , Qiaoyun Wu , Yiran Liu, Yufan He, Xiufang Hu, Xiaodan Zhang, Guohong Zeng

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引用次数: 0

Abstract

Aspergillus flavus is a saprophytic fungus that can infect economic crops and produce aflatoxins with high toxicity, carcinogenicity and mutagenicity, which is a serious threat to human and animal health. In order to find a more efficient biological control way to inhibit A. flavus and its production of aflatoxins, we isolated 7 fungal strains F1-F7 from Camellia sinensis. Only the strains F1 and F2, isolated from the flowers of C. sinensis and identified as Alternaria alstroemeriae and Alternaria burnsii, exhibited significant antagonistic activities against A. flavus. In addition, the contents of AFB1 and AFB2 significantly decreased when A. flavus was antagonized by Alternaria alstroemeriae F1. By the transcriptome and qRT-PCR analysis, some genes related to A. flavus growth and secondary metabolism were differentially expressed, and the expression levels of 14 genes in aflatoxins synthesis gene cluster that positively regulate aflatoxins synthesis all showed down-trends, and 2 genes (aflC and aflG) that negatively regulate aflatoxins synthesis showed up-trends. We hypothesize that the A. alstroemeriae F1 may secrete some secondary metabolites to inhibit the growth of A. flavus and decrease aflatoxins biosynthesis to a certain extent by regulating the expression levels of genes in aflatoxins biosynthesis gene cluster. In summary, this work lays a foundation for more effective biological controls against A. flavus growth and aflatoxins biosynthesis.

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黄曲霉F1抑制黄曲霉生长，抑制黄曲霉毒素合成

黄曲霉是一种腐生真菌，能侵染经济作物，产生具有高毒性、致癌性和诱变性的黄曲霉毒素，严重威胁着人类和动物的健康。为了寻找一种更有效的生物防治方法来抑制黄曲霉及其黄曲霉毒素的产生，我们从茶树中分离到了7株真菌F1-F7。只有从紫荆花中分离得到的菌株F1和F2对黄曲霉有明显的拮抗作用，鉴定为Alternaria alstroemeriae和Alternaria burnsii。AFB1和AFB2的含量在黄曲霉拮抗作用下显著降低。通过转录组和qRT-PCR分析，黄曲霉生长和次生代谢相关的部分基因存在差异表达，黄曲霉毒素合成基因簇中14个正向调控黄曲霉毒素合成的基因表达量均呈下降趋势，负向调控黄曲霉毒素合成的2个基因（aflC和aflG）表达量呈上升趋势。我们推测alstroemeriae F1可能通过调节黄曲霉毒素合成基因簇中基因的表达水平，分泌一些次生代谢物，在一定程度上抑制黄曲霉的生长，减少黄曲霉毒素的合成。本研究为更有效的生物防治黄曲霉生长和黄曲霉毒素的合成奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.