Integrating biochemical and functional assessments in equine joint research: A study of gait asymmetry and synovial fluid biomarkers

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-05-01 DOI:10.1016/j.jevs.2025.105486

L. Korac , L. St. George , N. Golestani , J. Souccar-Young , S. Topfer , S. Witherspoon , P. McCrae , A. Wildish , J. MacNicol , W. Pearson

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引用次数: 0

Abstract

Injection of recombinant interleukin-1β (reIL-1β) is commonly used in equine joint research to evaluate the clinical and biological outcomes of inflammation, often tracking changes over time to assess the effects of the experimental model. Incorporating functional assessments, such as gait asymmetry analysis, in conjunction with biological assessments, is essential for capturing the real-world impact of joint interventions and ensuring findings translate to meaningful improvements in equine health and locomotion. This study aimed to examine the relationship between synovial fluid biochemical changes and symmetry analysis (inertial measurement unit; IMU) following a 75-ng injection of recombinant interleukin-1β (reIL-1β) into the left intercarpal joint of 11 horses Synovial fluid samples were collected at 0 h (pre-injection; control) and at 6 h and 12 h post-injection and analyzed for prostaglandin E₂ (PGE₂), glycosaminoglycan (GAG), nitric oxide (NO), chondroitin sulfate epitope 846 (CS846), and resolvin D1 (RvD1). The IMU data were collected at baseline (hour 0) and 6 and 12 h post-injection to assess poll and pelvic movement during in-hand trot trials, with MinDiff (loading asymmetry) and MaxDiff (push-off asymmetry) values calculated. A linear mixed model was used to assess the association between biochemical outcomes and asymmetry measures with significance set at P ≤ 0.05. Increased synovial PGE₂ significantly contributed to greater poll MinDiff (estimate = 0.007, SE = 0.003, P = 0.006), whereas elevated CS846 was a key contributor to increased poll MaxDiff (estimate = 0.006, SE = 0.003, P = 0.036). Additionally, higher PGE₂ was linked to lower pelvis MaxDiff (estimate = −0.002, SE = 0.001, P = 0.024), whereas increased CS846 was associated with higher pelvis MinDiff (estimate = 0.003, SE = 0.001, P = 0.026). This research emphasizes the value of integrating biochemical and functional data to provide a more comprehensive tool for evaluating interventions for joint health.

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在马关节研究中整合生化和功能评估：步态不对称和滑液生物标志物的研究

重组白细胞介素-1β (reIL-1β)注射在马关节研究中常用来评估炎症的临床和生物学结果，经常跟踪随时间的变化来评估实验模型的效果。将功能评估（如步态不对称分析）与生物学评估结合起来，对于捕捉联合干预的实际影响并确保研究结果转化为对马健康和运动的有意义的改善至关重要。本研究旨在探讨滑液生化变化与对称性分析(惯性测量单元；IMU)注射重组白细胞介素-1β (reIL-1β) 75-ng后，于0 h(注射前；对照)和注射后6 h和12 h，分析前列腺素E2 （PGE2）、糖胺聚糖（GAG）、一氧化氮（NO）、硫酸软骨素表位846 （CS846）和溶解蛋白D1 （RvD1）。在基线（0小时）、注射后6和12小时收集IMU数据，以评估手部小跑试验期间的poll和骨盆运动，计算MinDiff（负荷不对称）和MaxDiff（推离不对称）值。采用线性混合模型评估生化结果与不对称测量之间的相关性，显著性设置为P≤0.05。滑膜PGE2显著增加了更大的调查MinDiff(估计 = 0.007 SE = 0.003,P = 0.006),而高CS846是一个关键因素增加调查MaxDiff(估计 = 0.006 SE = 0.003,P = 0.036)。此外,高PGE2与低骨盆MaxDiff(估计 = −0.002 SE = 0.001,P = 0.024),而增加CS846与更高的骨盆MinDiff(估计 = 0.003 SE = 0.001,P = 0.026)。本研究强调了整合生化和功能数据的价值，为评估关节健康干预措施提供了更全面的工具。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.