Yeast protein extraction assisted by Pulsed Electric Fields: Balancing electroporation and recovery

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-05-11 DOI:10.1016/j.foodhyd.2025.111527

Javier Marín-Sánchez, Alejandro Berzosa, Ignacio Álvarez, Javier Raso, Cristina Sánchez-Gimeno

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Abstract

Pulsed Electric Fields (PEF) technology is a promising method for extracting intracellular proteins from Saccharomyces cerevisiae by inducing membrane permeabilization. The degree of permeabilization, influenced by the number and size of pores formed, is expected to affect extraction efficiency. However, the impact of PEF treatment intensity on protein recovery remains unclear, particularly regarding the balance between membrane permeabilization and potential protein denaturation due to treatment. In this study, yeast cells were treated with PEF at 15 kV/cm across a total specific energy range (43.3–207.0 kJ/kg), and electroporation was assessed via flow cytometry. The release of amino acids, peptides, proteins, and protease activity was monitored over incubation time. The impact of field strength (5–20 kV/cm) on protein solubility was also analyzed. Lower-intensity treatments (43.3–84.0 kJ/kg) enabled up to 80 % protein recovery after 24 h, driven by protease activation and sustained hydrolysis. In contrast, higher-intensity treatments (≥121.1 kJ/kg) induced greater electroporation but reduced extraction efficiency (30–50 %) due to electric field–temperature synergy causing protein denaturation and loss of solubility. These findings highlight the need to optimize PEF conditions to balance electroporation and protein recovery, reinforcing PEF as a viable method for sustainable, high-quality protein recovery from yeast biomass.

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脉冲电场辅助酵母蛋白提取：平衡电穿孔和回收

脉冲电场（PEF）技术是一种很有前途的通过诱导膜渗透来提取酿酒酵母胞内蛋白的方法。渗透程度受形成的孔隙数量和大小的影响，预计会影响萃取效率。然而，PEF处理强度对蛋白质恢复的影响尚不清楚，特别是关于膜透性和处理导致的潜在蛋白质变性之间的平衡。在这项研究中，酵母细胞在15 kV/cm的总比能范围内（43.3-207.0 kJ/kg）用PEF处理，并通过流式细胞术评估电穿孔。氨基酸、多肽、蛋白质和蛋白酶活性的释放在孵育期间被监测。分析了电场强度（5 ~ 20 kV/cm）对蛋白质溶解度的影响。低强度处理（43.3-84.0 kJ/kg）在蛋白酶激活和持续水解的驱动下，24小时后蛋白质回收率高达80%。相比之下，高强度处理（≥121.1 kJ/kg）诱导了更大的电穿孔，但由于电场-温度协同作用导致蛋白质变性和溶解性丧失，提取效率降低（30 - 50%）。这些发现强调了优化PEF条件以平衡电穿孔和蛋白质回收的必要性，加强了PEF从酵母生物质中可持续、高质量地回收蛋白质的可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.