Bioactive pterostilbene encapsulated in zein via an in-situ co-precipitation strategy for efficient antimicrobial and fruit preservative

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-05-09 DOI:10.1016/j.foodhyd.2025.111529

Chunli Fang , Zhinan Fu , Nan Wei , Jia Wei , Shuying Li , Ting Feng , Zicheng Tan , Junyou Wang , Bin Wu , Xuhong Guo

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引用次数: 0

Abstract

Pterostilbene (PTS) mainly originated from grapes and palm trees, is a promising bioactive phytoalexin, as it possesses excellent activity against the food-deteriorating microorganisms. For effective use in food preservation, stable encapsulation of a large amount of low-soluble PTS within biomaterials is crucial. In this work, plant-sourced zein macromolecule was used for the efficient encapsulation of PTS (PTS-loaded NPs) via an in-situ co-precipitation strategy for the first time. Compared with conventional stirring precipitation methods, this flow-based new approach demonstrated superior encapsulation efficiency (>93 %) and controllable operating mode. By regulating fluid-involved parameters such as feeding components and flow rates, the particle size, size distribution, and PTS loading efficiency could be easily adjusted. The optimal PTS-loaded NPs demonstrated exceptional storage stability (>14 days), prolonged release property (>48 h), and enhanced antioxidant activity. Additionally, the PTS-loaded NPs exhibited strong antimicrobial action, reducing the growth of A. niger by 50 % and that of E. coli and S. aureus by 99 % when compared to the control. Profiting from the food-grade biomaterial, the non-toxic PTS-loaded NPs enhanced the shelf life of strawberries over 3 days when compared to control with respect to color, weight loss, firmness, and total soluble solids.

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玉米蛋白原位共沉淀法包封具有生物活性的紫油二苯乙烯，用于高效抗菌和水果防腐

紫檀芪（Pterostilbene， PTS）主要来源于葡萄和棕榈树，是一种很有前景的生物活性植物抗菌素，对食品变质微生物具有良好的抑制作用。为了有效地在食品保存中使用，在生物材料中稳定地封装大量低溶PTS是至关重要的。本研究首次利用植物源玉米蛋白大分子通过原位共沉淀法对PTS（负载的NPs）进行高效包封。与传统的搅拌沉淀方法相比，这种基于流动的新方法具有更高的封装效率（> 93%）和可控的操作模式。通过调节进料组分和流量等与流体有关的参数，可以很容易地调节颗粒大小、粒度分布和PTS加载效率。最佳的pts负载NPs表现出优异的储存稳定性（14天），延长的释放特性（48小时）和增强的抗氧化活性。此外，与对照相比，负载pts的NPs表现出强大的抗菌作用，使黑曲霉的生长减少50%，大肠杆菌和金黄色葡萄球菌的生长减少99%。受益于食品级生物材料，与对照相比，无毒的pts负载NPs在颜色，重量减轻，硬度和总可溶性固形物方面延长了草莓的保质期超过3天。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.