Sustainable extraction and characterization of type I collagen from tuna skin waste utilizing biocompatible ionic liquid-based aqueous two-phase system

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-05-07 DOI:10.1016/j.foodhyd.2025.111524

Qianqian Chen , Botao Liang , Xuan Yuan, Xinyi Yu, Chengcheng Li, Lai Wei, Jing Ye, Jiajia Wu, Zhiyuan Dai, Yanbin Lu

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引用次数: 0

Abstract

In this study, a green method for collagen recovery from tuna processing waste utilizing a biocompatible ionic liquid-based aqueous two-phase system (ATPS) was established. The phase behavior of selected extractants, including choline-based ionic liquids (Ch-ILs) and the non-ionic surfactant Triton X-100 (TX-100), was systematically investigated and evaluated based on their phase diagrams, density functional theory calculations, and nuclear magnetic resonance analysis. Through optimization of single-factor experiments, the optimal ATPS was determined to consist of TX-100, [Ch][DHC], and water in a weight ratio of 16:30:54. Following ultrasonic treatment for 2 h at a solid-to-liquid ratio of 1:10, an average collagen yield of 16.61 ± 0.30 % was achieved without requiring tedious sample pretreatment steps. The purified collagen was characterized and validated through a series of spectral analyses, confirming that it maintained a relatively intact triple helix structure. It is noteworthy that the recovery and recycling rates for both [Ch][DHC] and TX-100 were evaluated during the extraction process, with results indicating that these rates exceeded 87.5 %. Additionally, the potential extraction mechanisms underlying the interactions between collagen and the studied ionic liquids were investigated using molecular docking simulations. This proposed extraction strategy aligns with principles of being environmentally friendly, safe, and sustainable—thereby enhancing its application prospects for effective treatment of tuna processing waste.

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利用生物相容性离子液体基双水相体系从金枪鱼皮废弃物中可持续提取I型胶原蛋白并进行表征

本研究建立了一种利用生物相容性离子液体基双水相系统（ATPS）从金枪鱼加工废料中回收胶原的绿色方法。基于相图、密度泛函理论计算和核磁共振分析，系统地研究了抽取剂的相行为，包括基于胆碱的离子液体（Ch-ILs）和非离子表面活性剂Triton X-100 （TX-100）。通过单因素实验优化，确定最佳的ATPS为TX-100、[Ch][DHC]和水，质量比为16:30:54。以1:10的料液比超声处理2小时后，无需繁琐的样品前处理步骤，平均胶原得率为16.61±0.30%。纯化后的胶原蛋白通过一系列的光谱分析进行了表征和验证，证实其保持了相对完整的三螺旋结构。值得注意的是，在提取过程中对[Ch][DHC]和TX-100的回收率和再利用率进行了评价，结果表明[Ch][DHC]和TX-100的回收率均超过87.5%。此外，利用分子对接模拟研究了胶原蛋白与离子液体相互作用的潜在提取机制。提出的提取策略符合环境友好、安全和可持续的原则，从而提高了其在有效处理金枪鱼加工废物方面的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.