Dental adhesives incorporated with alkyl trimethyl ammonium bromide-loaded titanium oxide nanotubes for sustained bioactive and anti-biofilm protection

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-04-17 DOI:10.1016/j.dental.2025.04.002

Isadora Martini Garcia , Vitaliano Gomes Araújo-Neto , Gabriela Balbinot , Virgínia Serra Souza , Lamia Mokeem , Jackson Damiani Scholten , Mary Anne Sampaio Melo , Marcelo Giannini , Fabrício Mezzomo Collares

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引用次数: 0

Abstract

Objectives

This study aimed to formulate experimental dental adhesives with alkyl trimethyl ammonium bromide-loaded titanium oxide nanotubes (ntTiO₂/ATAB) and evaluated their physicochemical properties, antimicrobial activity, mineral deposition, and cytotoxicity.

Methods

ntTiO₂/ATAB was synthesized, characterized, and added into an experimental adhesive resin. The filler ntTiO₂/ATAB was added at 1, 2.5, and 5 wt% (G_1 %, G_2.5 %, G_5 %) in the adhesive. A control group (G_0 %) without ntTiO₂/ATAB was also prepared and used in all tests. The adhesives were analyzed for degree of conversion (DC%), softening in solvent, radiopacity, antibacterial activity against saliva-derived microcosm, mineral deposition capability, and cytotoxicity.

Results

Analytical techniques, including TEM, FTIR, UV-Vis, and micro-Raman spectroscopy, confirmed the structure and chemical composition of the ntTiO₂/ATAB. A DC% over 60 % was observed for all groups, with no adverse impact on radiopacity or softening in solvent. Antibacterial testing indicated that increasing ntTiO₂/ATAB concentration led to reduced colony-forming units of critical microorganisms, with this effect sustained over a one-year aging period. Mineral deposition tests showed enhanced phosphate presence over the samples with higher ntTiO₂/ATAB concentrations. There were no statistical differences in human cell viability among groups.

Significance

The dental adhesives formulated with ntTiO₂/ATAB demonstrated suitable physical and chemical properties, including reliable bond strength to dentin previously tested. They also exhibited antibacterial effects against caries-related microorganisms even after aging, promoted bioactivity through mineral deposition, and showed no cytotoxicity against human cells. These adhesives represent a promising strategy to assist in reducing the risk of recurrent caries and preserve the material-dentin interface properties over time.

查看原文本刊更多论文

牙科胶粘剂与负载烷基三甲基溴化铵的氧化钛纳米管结合，具有持续的生物活性和抗生物膜保护

目的制备烷基三甲基溴化铵负载氧化钛纳米管（ntTiO2/ATAB）牙用胶粘剂，并对其理化性能、抗菌活性、矿物沉积和细胞毒性进行评价。方法合成snttio_2 /ATAB，对其进行表征，并将其加入到实验粘接树脂中。在胶粘剂中分别添加1、2.5、5 wt% （G1 %,G2.5 %,G5 %）的填料ntTiO₂/ATAB。同时制备不含ntTiO 2 /ATAB的对照组（G0 %），用于所有试验。分析了胶粘剂的转化率（DC%）、溶剂软化、不透光性、对唾液微生物的抗菌活性、矿物沉积能力和细胞毒性。结果TEM、FTIR、UV-Vis、微拉曼光谱等分析技术证实了nttio2 /ATAB的结构和化学成分。所有组的DC%均大于60 %，对放射不透性或溶剂软化无不利影响。抗菌测试表明，增加ntTiO 2 /ATAB浓度导致关键微生物的集落形成单位减少，这种效果持续了一年的老化期。矿物沉积试验表明，在具有较高的ntTiO 2 /ATAB浓度的样品中，磷酸盐的存在增强。各组间人体细胞活力差异无统计学意义。用ntTiO2/ATAB配制的牙胶粘剂表现出合适的物理和化学性能，包括与先前测试的牙本质的可靠结合强度。即使在衰老后，它们也表现出对龋齿相关微生物的抗菌作用，通过矿物质沉积促进生物活性，并且对人体细胞没有细胞毒性。这些粘接剂代表了一种很有前途的策略，可以帮助降低复发性龋齿的风险，并随着时间的推移保持材料-牙本质界面的特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.