Selective RNA binding and imaging with imidazopyrazine-based fluorescent molecule

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-10 DOI:10.1016/j.saa.2025.126382

Zekiye Şeyma Sevinçli , Karina Amudi , Buse Ceyda Öncel , Erkan Yurtcu , Özlem Darcansoy İşeri , Nurettin Menges

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引用次数: 0

Abstract

We report the synthesis and characterization of novel imidazopyrazine-based fluorescent molecules 5a and 5b targeting RNA and DNA binding. Molecule 5b showed superior photophysical properties with stable fluorescence and high quantum yield in various solvents. UV–Vis and fluorescence spectroscopy revealed strong RNA binding with time-dependent fluorescence quenching and increasing absorbance, suggesting groove binding or π-π stacking interactions. Furthermore, agarose gel electrophoresis further confirmed selective RNA binding of 5b. Imaging studies demonstrated that 5b penetrated into viable MCF-7 cells and selectively stained RNA and retained fluorescence for up to 8 h under ambient conditions. These findings advance the study of RNA dynamics in living cells, highlighting the potential of 5b for RNA-specific bioimaging and sensing applications.

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基于咪唑吡嗪的荧光分子的选择性RNA结合和成像

我们报道了基于咪唑吡嗪的新型靶向RNA和DNA结合的荧光分子5a和5b的合成和表征。分子5b表现出优异的光物理性质，在各种溶剂中具有稳定的荧光和高量子产率。紫外-可见光谱和荧光光谱显示，RNA结合强烈，荧光猝灭，吸光度增加，可能是凹槽结合或π-π堆叠相互作用。琼脂糖凝胶电泳进一步证实了5b的选择性RNA结合。成像研究表明，5b能穿透活的MCF-7细胞，选择性地染色RNA，并在环境条件下保持长达8小时的荧光。这些发现促进了活细胞中RNA动力学的研究，突出了5b在RNA特异性生物成像和传感应用中的潜力。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.