Detection of mitophagy in live cells with indole derived near-infrared fluorogenic probes

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-10 DOI:10.1016/j.saa.2025.126368

Xuanyuan Wang , Wen Chen , Shuangling Liu , Yihong Xu , Zhimei Xiong , Yingzi Li , Leyuan Huang , Lu Jiang , Jingting Zhang , Leying Sun , Yuan Zhang , Mengqin Liu

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引用次数: 0

Abstract

Mitophagy is an indispensable cellular process that plays a crucial role in regulating mitochondrial quality control and cellular metabolism. Therefore, monitoring the changes in the mitochondrial and lysosomal microenvironment during the mitophagy process is extremely important. However, existing mitophagy probes only target changes in a single indicator (viscosity, pH value, or polarity) within the microenvironment, which may reduce the selectivity and accuracy of assessing mitophagy in complex biological settings. To address this, we have developed a dual-channel detection near-infrared (NIR) fluorescent probe (ADMI). In vitro analysis experiments have shown that ADMI not only responds to pH and activates the NIR fluorescence channel but also that the green fluorescence channel exhibits high sensitivity to changes in polarity. This dual-response mechanism probe enables dual fluorescent detection of pH and polarity, providing a highly promising tool for monitoring the microenvironment of mitophagy in living cells. Ultimately, we applied ADMI to real-time monitoring of mitophagy induced by starvation or rapamycin, during which the decrease in pH and polarity resulted in a red shift in wavelength and increased fluorescence. Additionally, ADMI was able to observe changes in mitochondria during ferroptosis. This probe may serve as a useful tool for imaging mitophagy in living cells.

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用吲哚衍生近红外荧光探针检测活细胞的有丝分裂

线粒体自噬是一个不可缺少的细胞过程，在调节线粒体质量控制和细胞代谢中起着至关重要的作用。因此，监测线粒体和溶酶体微环境在线粒体自噬过程中的变化是非常重要的。然而，现有的线粒体自噬探针仅针对微环境中单一指标（粘度、pH值或极性）的变化，这可能会降低在复杂生物环境中评估线粒体自噬的选择性和准确性。为了解决这个问题，我们开发了一种双通道检测近红外荧光探针（ADMI）。体外分析实验表明，ADMI不仅响应pH值，激活近红外荧光通道，而且绿色荧光通道对极性变化具有很高的敏感性。这种双响应机制探针可以实现pH和极性的双荧光检测，为监测活细胞中有丝分裂的微环境提供了一种非常有前途的工具。最后，我们将ADMI应用于饥饿或雷帕霉素诱导的线粒体自噬的实时监测，在此过程中，pH和极性的降低导致波长红移和荧光增加。此外，ADMI能够观察到铁下垂期间线粒体的变化。该探针可作为成像活细胞有丝分裂的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.