Construction of a ratiometric turn-on fluorescent probe based on amino acid skeleton for specific detection of Al3+ ions in environmental and biological system

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-09 DOI:10.1016/j.saa.2025.126364

Yuchen Lin , Haiyan Xu , Wenlong Qin , Ying-Guo Liu

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引用次数: 0

Abstract

In this work, a novel colorimetric and fluorometric dual-channel fluorescent sensor NHO based on an unsaturated amino acid derivative was rationally designed. The sensor exhibited discerning and expeditious fluorescence sensing capabilities towards Al³⁺ in MeOH/Hepes buffer solution (9/1, v/v, pH 7.4). The fluorescence of NHO at 537 nm was significantly and instantaneously enhanced upon addition of Al³⁺ ions, and this fluorescence could be reversibly quenched by EDTA. The detection limit (LOD) and binding constant of NHO for Al³⁺ were determined to be 24.8 nM and 6.1 x 10⁹ M⁻², respectively. The mechanism of interaction between NHO and Al³⁺ was comprehensively investigated and theoretically supported via DFT calculation. Furthermore, NHO’s multifaceted practical applications in detecting Al³⁺ ions were explored, including tests in real water samples, commercially available almagate suspension and applications on test paper. A smartphone-assisted detection platform was developed, making the monitoring of Al³⁺ ions more convenient and cost-effective. Additionally, NHO also exhibited promising bioimaging capabilities in PK-15 cells. These inventions highlighted that NHO was a useful tool for the detection of Al³⁺ in both environmental and living systems.

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基于氨基酸骨架的比例开启荧光探针的构建，用于环境和生物系统中Al3+离子的特异性检测

本文合理设计了一种基于不饱和氨基酸衍生物的新型比色荧光双通道荧光传感器NHO。该传感器对MeOH/Hepes缓冲溶液（9/1,v/v, pH 7.4）中的Al3+具有识别和快速的荧光传感能力。添加Al3+离子后，NHO在537 nm处的荧光瞬间显著增强，EDTA可可逆猝灭这种荧光。NHO对Al3+的检出限（LOD）和结合常数分别为24.8 nM和6.1 × 109 M−2。通过DFT计算，对NHO与Al3+的相互作用机理进行了全面的研究和理论支持。此外，还探讨了NHO在检测Al3+离子方面的多方面实际应用，包括在真实水样中的测试、市售的藻酸盐悬浮液和在试纸上的应用。开发了智能手机辅助检测平台，使Al3+离子的监测更加便捷和经济。此外，NHO在PK-15细胞中也表现出了很好的生物成像能力。这些发明突出表明，NHO是检测环境和生命系统中Al3+的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.