Anaerobic bacteraemia - identification of Bacteroides and Phocaeicola in blood samples. Challenges in antimicrobial susceptibility testing

The Microbe Pub Date : 2025-05-10 DOI:10.1016/j.microb.2025.100374

Marta Kierzkowska , Kinga Markowska , Jan Kownacki , Edyta Podsiadły , Anna Majewska

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Abstract

The objective of this retrospective, single-centre, observational study was to determine the prevalence of Gram-negative anaerobic bacteria, specifically Bacteroides, and Phocaeicola in blood samples taken from patients with bacteremia, to describe the antibiotic susceptibility profiles of these clinical isolates, and to investigate the presence of resistance genes. Bacteroides fragilis was the most frequently isolated from blood samples, accounting for 64 % of cases. Antibiotic susceptibility testing revealed different patterns of resistance to antimicrobial agents. Almost all (96 %) strains carried genes associated with resistance to β-lactam antibiotics, in a mechanism related to enzyme production. Phenotypic susceptibility to amoxicillin with clavulanic acid and ticarcillin was observed in 92 % and 72 % of the isolates, respectively. All the strains were susceptible to metronidazole, and the nim gene was not detected. The erm genes were detected in 24 % of the strains, all of which were phenotypically resistant to clindamycin.

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厌氧菌血症——血液样本中拟杆菌和Phocaeicola的鉴定。抗微生物药敏试验的挑战

这项回顾性、单中心、观察性研究的目的是确定从菌血症患者的血液样本中革兰氏阴性厌氧菌，特别是拟杆菌和Phocaeicola的患病率，描述这些临床分离株的抗生素敏感性概况，并调查耐药基因的存在。易碎拟杆菌（Bacteroides fragilis）最常从血液样本中分离出来，占64 %。抗生素药敏试验揭示了不同的抗微生物药物耐药模式。几乎所有（96 %）菌株都携带与β-内酰胺类抗生素耐药相关的基因，其机制与酶的产生有关。克拉维酸和替卡西林对阿莫西林的表型敏感性分别为92% %和72% %。所有菌株均对甲硝唑敏感，未检出nim基因。在24% %的菌株中检测到erm基因，所有菌株都对克林霉素具有表型抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Microbe

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