Enhancing fatty acid production from waste activated sludge through low-temperature heat treatment and biostimulant pretreatment: Performance and mechanism analysis

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-05-12 DOI:10.1016/j.bej.2025.109789

Yuan Shan , Yanyan Wang , Jing Zhang , Yihan Gong , Tiantian Yao , Yihao Wang , Guanghui Chen , Deshuang Yu

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Abstract

Anaerobic fermentation of sludge is constrained by low short-chain fatty acids (SCFAs) yields and limited solubilization of organic matter. This study presents a novel approach that integrates low-temperature heat treatment with biostimulant pretreatment to enhance the recovery of SCFAs from waste activated sludge. Experimental results indicate that applying 90 °C heat treatment and 1.2 g/g TSS D-limonene without pH adjustment led to an SCFAs yield of 2664 mg/L, representing a 2.3-fold increase over the control group. Furthermore, under conditions with initial pH set to 12, the SCFAs yield peaked at 2977 mg/L by the seventh day. Mechanistic analysis revealed that the combined pretreatment effectively disrupted extracellular polymeric substances and cell walls, facilitating SCFAs production. Microbial community analysis demonstrated an enrichment of Proteobacteria, Actinobacteria, and chain-elongating bacteria, enhancing the conversion of low-value organic matter into high-value products. The results offer new perspectives for optimizing anaerobic fermentation processes to enhance organic matter recovery from sludge.

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通过低温热处理和生物刺激素预处理提高废活性污泥脂肪酸产量：性能及机理分析

污泥的厌氧发酵受到低短链脂肪酸（SCFAs）产量和有限的有机物增溶的限制。本研究提出了一种将低温热处理与生物刺激素预处理相结合的新方法，以提高废弃活性污泥中scfa的回收率。实验结果表明，在不调整pH的情况下，经90°C热处理和1.2 g/g TSS d -柠檬烯处理，SCFAs产率为2664 mg/L，比对照组提高2.3倍。在初始pH为12的条件下，第7天SCFAs产量达到2977 mg/L。机制分析表明，联合预处理有效地破坏了细胞外聚合物物质和细胞壁，促进了scfa的产生。微生物群落分析表明，变形菌、放线菌和长链细菌的富集，促进了低价值有机物向高价值产品的转化。研究结果为优化厌氧发酵工艺以提高污泥有机物回收率提供了新的视角。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.