Increased locomotor activity does not mitigate the effects of advanced maternal age in a mouse model

Abstract

Introduction

Advanced maternal age (AMA) increases the risk of pregnancy complications, in part due to impaired placentation. While exercise during pregnancy can improve outcomes, its potential to mitigate the effects of AMA has not been investigated. We evaluated the impact of exercise in a mouse model of AMA.

Methods

Females were paired with males at 9 or 34 weeks of age, with one group of aged females having access to running wheels four weeks prior to and during pregnancy. Pregnant females (N = 19 per group) were collected at gestational day (GD) 11.5. Placentas were collected for RNA sequencing (N = 17–20 per group).

Results

Aged females with access to running wheels had lighter fat depots (1.0 ± 0.1 g) than those without (2.2 ± 0.1 g; p < 0.0001), but did not differ from young females (0.8 ± 0.1 g; p = 0.5). Both groups of aged females had fewer viable conceptuses (without wheels: 4.0 ± 0.5, with wheels: 4.3 ± 0.5) than young mice (8.3 ± 0.5; p < 0.0001 for both comparisons). Fetal crown-rump length was also lower in aged females (without wheels: 5.7 ± 0.2 mm, with wheels: 5.5 ± 0.2 mm, young: 6.6 ± 0.2 mm; p < 0.0001 for both comparisons). Placental expression of only one gene was affected by access to running wheels, but 423 and 967 genes were differentially expressed between young and aged females without and with access to wheels, respectively. Placental transcriptomes suggested delayed placental development in aged females.

Conclusions

Our model reproduced previously-reported effects of age on fetal development and placental transcriptomics, but these were not mitigated by increased voluntary locomotor activity, despite a reduction in adiposity. Remarkably, increased voluntary locomotor activity had almost no effects on placental gene expression in aged mice.