{"title":"Single-Atom Colloidal Nanorobotics Enhanced Stem Cell Therapy for Corneal Injury Repair.","authors":"Xiaohui Ju,Eliška Javorková,Jan Michalička,Martin Pumera","doi":"10.1021/acsnano.4c18874","DOIUrl":null,"url":null,"abstract":"Corneal repair using mesenchymal stem cell therapy faces challenges due to long-term cell survival issues. Here, we design cerium oxide with gold single-atom-based nanorobots (CeSAN-bots) for treating corneal damage in a synergistic combination with stem cells. Powered by glucose, CeSAN-bots exhibit enhanced diffusion and active motion due to the cascade reaction catalyzed by gold and cerium oxide. CeSAN-bots demonstrate a two-fold increase in cellular uptake efficiency into mesenchymal stem cells compared to passive uptake. CeSAN-bots possess intrinsic antioxidant and immunomodulatory properties, promoting corneal regeneration. Validation in a mouse corneal alkali burn model reveals an improvement in corneal clarity restoration when stem cells are incorporated with CeSAN-bots. This work presents a strategy for developing glucose-driven, enzyme-free, single-atom-based ultrasmall nanorobots with promising applications in targeted intracellular delivery in diverse biological environments.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"57 1","pages":""},"PeriodicalIF":15.8000,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.4c18874","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Corneal repair using mesenchymal stem cell therapy faces challenges due to long-term cell survival issues. Here, we design cerium oxide with gold single-atom-based nanorobots (CeSAN-bots) for treating corneal damage in a synergistic combination with stem cells. Powered by glucose, CeSAN-bots exhibit enhanced diffusion and active motion due to the cascade reaction catalyzed by gold and cerium oxide. CeSAN-bots demonstrate a two-fold increase in cellular uptake efficiency into mesenchymal stem cells compared to passive uptake. CeSAN-bots possess intrinsic antioxidant and immunomodulatory properties, promoting corneal regeneration. Validation in a mouse corneal alkali burn model reveals an improvement in corneal clarity restoration when stem cells are incorporated with CeSAN-bots. This work presents a strategy for developing glucose-driven, enzyme-free, single-atom-based ultrasmall nanorobots with promising applications in targeted intracellular delivery in diverse biological environments.
期刊介绍:
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.