Direct monitoring of electrochemical behavior of viable E. coli under various stress conditions without mediators

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-12 DOI:10.1016/j.bios.2025.117578

Xinfang Zhang , Kai Zhou , Xian Mao , Ying Xiong , Jiali Ren

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Abstract

Escherichia coli (E. coli) plays a vital role in human life and various fields, yet its naturally non-electroactive nature presents challenges for electrochemical detection. In this study, we directly monitored E. coli's electrochemical behavior in an M9 medium without exogenous electron shuttles or genetic modifications, obtaining an oxidation peak at +0.35 V (vs Ag/AgCl). The electrochemical signal correlated with bacterial growth and viability. Under stress conditions (hypoxia, acid, heat, osmotic, oxidative, and metal ion stress), signal intensity correlates with the number of viable E. coli cells and their electron transport activity. Hydroquinone (HQ) was identified as the contribution to the signal via electrochemical analysis, Prep-HPLC, and GC-MS. This study directs the detection of quinone-related electrochemical behavior in E. coli, offering insights into quinone-mediated electron transfer and potential applications in food science and environmental engineering.

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在无介质条件下直接监测不同胁迫条件下活菌的电化学行为

大肠杆菌在人类生活和各个领域发挥着至关重要的作用，但其天然的非电活性给电化学检测带来了挑战。在本研究中，我们直接监测了大肠杆菌在无外源电子穿梭或基因修饰的M9培养基中的电化学行为，获得了+0.35 V （vs Ag/AgCl）时的氧化峰。电化学信号与细菌生长和生存能力相关。在胁迫条件下（缺氧、酸、热、渗透、氧化和金属离子胁迫），信号强度与存活的大肠杆菌细胞数量及其电子传递活性相关。通过电化学分析、制备高效液相色谱和气相色谱-质谱分析确定对苯二酚（HQ）对信号的贡献。本研究指导了大肠杆菌中醌类相关的电化学行为的检测，为醌介导的电子转移及其在食品科学和环境工程中的潜在应用提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.