Impact of spawning season on sperm production and motility in northern pike (Esox lucius): A comparison of stripped and testicular sperm

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-05-11 DOI:10.1016/j.aquaculture.2025.742695

Beata Irena Cejko , Sławomir Krejszeff , Agata Anna Cejko , Michał Dubrowski

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引用次数: 0

Abstract

We previously demonstrated that, although there were significant differences in reproductive efficiency (semen volume, sperm concentration, total sperm count) between wild northern pike (Esox lucius) stripped semen (SS) and testicular semen (TS), their motility and kinematic parameters remained similar immediately after collection and after 24 h of short-term storage in an undiluted state during the spawning season (i.e., mid-March). However, longer semen storage of up to 72 h led to decreases in motility and kinematic parameters in SS compared to TS. This indicated that northern pike semen obtained from spermatic ducts lost its biological value (fertilization capacity) much faster than that obtained from testes. Here, for the first time, we examined the effect of the spawning season at the beginning (March 22), in the middle (March 30), and at the end (April 13) on the quantity, quality, and motility of wild northern pike SS and TS. The SS total volume (TVS; ml), volume per kg of body weight (VOS; ml kg⁻¹ b.w.), sperm concentration (SC; ×10⁹ ml⁻¹), and sperm count, i.e., total sperm production (TSP; ×10⁹), and total number of sperm per kg of body weight (TNS; ×10⁹ kg⁻¹b.w.) remained constant throughout the spawning season, suggesting stable production regardless of the stage of the season. In contrast, TS exhibited significant decreases in VOS, SC, TSP, and TNS as spawning progressed. The highest values were recorded at the beginning of the season, while no differences between SS and TS were noted at the end of the season. SS had higher motility values at the beginning of the season, but these parameters deteriorated rapidly as the season progressed. In contrast, TS showed greater stability in sperm motility and velocity parameters throughout the spawning season. This stability may be important for management strategies of northern pike reproduction, both in the wild and in breeding programs.

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产卵季节对北方梭子鱼精子产生和活力的影响：剥离精子和睾丸精子的比较

我们之前的研究表明，尽管野生北梭子鱼（Esox lucius）剥脱精液（SS）和睾丸精液（TS）在生殖效率（精液体积、精子浓度、总精子数）方面存在显著差异，但它们的运动和运动学参数在采集后立即保持相似，在产卵季节（即3月中旬）在未稀释状态下短期储存24小时后保持相似。然而，与TS相比，精液储存时间长达72小时，导致SS的运动和运动学参数下降，这表明从精管中获得的北梭子鱼精液比从睾丸中获得的精子更快地失去其生物学价值（受精能力）。本研究首次考察了产卵季节初期（3月22日）、中期（3月30日）和后期（4月13日）对野生梭子鱼SS和TS的数量、质量和活动性的影响。ml)，每千克体重体积(VOS；ml kg−1 b.w)，精子浓度(SC；×109 ml−1)和精子数量，即总精子产量(TSP；×109)，以及每公斤体重的精子总数(TNS；×109 kg−1b.w.)在整个产卵季节保持不变，这表明无论季节的哪个阶段，产量都是稳定的。相反，随着产卵的进行，TS的VOS、SC、TSP和TNS显著降低。最高的值记录在季初，而在季末，SS和TS之间没有差异。SS在季初具有较高的活动力值，但随着季节的进展，这些参数迅速恶化。相比之下，TS在整个产卵季节精子活力和速度参数方面表现出更大的稳定性。这种稳定性可能对北梭子鱼繁殖的管理策略很重要，无论是在野外还是在繁殖计划中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.