Construction and application of a NIR benzothianone-based ratiometric fluorescent probe for visual detection of Hg2+ in biological systems and water samples

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-05-12 DOI:10.1016/j.aca.2025.344176

Huan Zhang, Haoyu Wu, Limeng Cong, Aojie Dai, Ning Qu, Shuai Guo, Songhua Zhu, Xiangbo Gou, Youlai Zhang

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引用次数: 0

Abstract

Background

Mercury ions, a toxic heavy metal, pose a significant threat to environmental health, leading to pollution and accumulation in living organisms.

Results

In this study, we have developed a novel ratiometric near-infrared fluorescent probe for the detection of Hg²⁺. The probe TM-ND-Hg demonstrated a remarkable Stokes shift (358 nm), a rapid response time (within 1 min), excellent selectivity, stability, and ratiometric fluorescence changes from green to red. Moreover, the probe enabled the portable detection of Hg²⁺ through color recognition software, which can discern color alterations.

Significance

The probe has been successfully applied to monitor Hg²⁺ in living A549 cells, zebrafish, and real water samples, showcasing its potential for environmental and biological monitoring.

Abstract Image

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基于苯并噻吩酮的近红外比例荧光探针的构建与应用，用于生物系统和水样中Hg2+的视觉检测

汞离子是一种有毒的重金属，对环境健康构成重大威胁，导致生物体内的污染和积累。结果在本研究中，我们开发了一种用于检测Hg2+的新型比例荧光探针。该探针TM-ND-Hg具有显著的Stokes位移（358nm）、快速的响应时间（1分钟内）、出色的选择性、稳定性和从绿色到红色的比例荧光变化。此外，探头通过颜色识别软件实现了Hg2+的便携式检测，该软件可以识别颜色变化。该探针已成功应用于A549活细胞、斑马鱼和真实水样中的Hg2+监测，显示了其在环境和生物监测方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.