Insights into the drivers of antibiotic resistance gene migration in soil-lettuce system with manure application from different sources

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-05-13 DOI:10.1016/j.envpol.2025.126444

Hunan Liu , Lanjun Wang , Zikun Dong , Shengfang Wen , Changrui Liu , Junpu Wang , Jun Wang , Lusheng Zhu , Young Mo Kim , Jinhua Wang

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Abstract

The application of livestock manure serves as a significant source of ARGs in soils. To study the impact of manure application on the migration of ARGs in the soil-plant system, we set different application ratios (1 %, 3 %, 8 %) of chicken and cow manure for treatment. The study's results demonstrated that the application of organic fertilizers increased the quantity of resistance genes in soil, root, and leaf zones. This change was influenced by the type and proportion of the organic fertilizers used. ARGs and MGEs exhibited the highest absolute enrichment levels in leaf tissues (2.53 and 2.01 times, respectively) with the 3 % cow manure treatment. In contrast, chicken manure exhibited the highest enrichment levels after the 1 % addition (2.51 and 1.81 times, respectively). The applied manure exhibited a high degree of similarity in bacterial community composition with the soil-lettuce system, indicating that ARGs may spread through microorganisms in this system. This study demonstrated that the evolution of bacterial community structure plays a pivotal role in mediating and driving the migration of ARGs within manure-amended soil-plant ecosystems, providing a theoretical basis for understanding the transmission of ARGs in soils and plants due to agricultural production activities.

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不同来源肥料对土壤-生菜系统中抗生素抗性基因迁移驱动因素的研究

畜禽粪便的施用是土壤中ARGs的重要来源。为了研究施用有机肥对土壤-植物系统中ARGs迁移的影响，我们设置了不同施用比例（1%、3%、8%）的鸡粪和牛粪进行处理。研究结果表明，施用有机肥增加了土壤、根区和叶区的抗性基因数量。这种变化受施用有机肥的种类和比例的影响。在3%牛粪处理下，叶片组织中ARGs和MGEs的绝对富集量最高，分别为2.53倍和2.01倍。而添加1%后，鸡粪的富集水平最高（分别为2.51倍和1.81倍）。施用有机肥与土壤-生菜系统的细菌群落组成高度相似，表明ARGs可能通过该系统中的微生物传播。该研究表明，细菌群落结构的进化在土壤-植物生态系统中对ARGs的迁移起着关键的调节和驱动作用，为理解农业生产活动导致的ARGs在土壤和植物中的传播提供了理论基础。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.