Selenium Distribution and Speciation in Tissues from Rats Administered with Non-Native Selenotrisulfides

Abstract

Selenotrisulfides (STS, R–S–Se–S–R) are metabolic intermediates in the bioconversion of inorganic Se species to organoselenium compounds. These Se species are reactive with a variety of endogenous molecules, particularly thiol-containing proteins, with this reactivity facilitating Se transport and subsequent utilization within the body. In this study, X-ray fluorescence microscopy (XFM) and high energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) were applied to investigate Se distribution and speciation in vivo. Male rats administered with 1 mg Se/kg b.w. as selenious acid (SA), L-penicillamine selenotrisulfide (PenSSeSPen) or selenenyl penicillamine bound to rat serum albumin (RSA-SSeSPen) showed statistically significant elevations in Se concentrations in the kidney, liver, and blood after 48 h treatment; however, no change in Se concentration was observed in the testes. Notably, XFM revealed a strong colocalization of Se and Cu in the renal cortex, a phenomenon previously observed in cultured cells and in rats fed diets supplemented with 5 mg Se/kg as selenite. Linear combination and principal component analyses of Se Kα₁ HERFD-XAS spectra revealed marked differences in Se speciation between the renal cortex and medulla and between red blood cells and plasma for all groups, including the control. STS were identified in linear combination fits of spectra from all tissues, except the testes. These results highlight the vital roles of STS in the intracellular reduction and transport of Se throughout the bloodstream and various tissues.