Salinity-Induced Gene Expressions in Pangasius nasutus (Bleeker, 1863)

IF 1.9 4区 农林科学 Q2 FISHERIES
Mohamad Ali Nabilah, Victor Tosin Okomoda, Danish Daniel Abdullah Muhd, Asma Ariffin Nur, Sheriff Md. Shahreza
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Abstract

This study was designed to evaluate the molecular responses of Pangasius nasutus to salinity exposure of varying concentrations. Juveniles of P. nasutus from similar breeding history (average size of 35 ± 0.54 cm and 152 ± 1.82 g) were obtained from a known source in Terengganu. The salinity range used for this study (i.e., 0, 10, 15, and 20 ppt) was determined through a range finding test, and the juveniles of P. nasutus were exposed in triplicates to these selected salinity ranges for 2 weeks under laboratory conditions. Thereafter, muscle tissues were collected from 10 biological replicates for molecular analysis. Differential display reverse transcriptase PCR was used to identify the expressed cDNA fragments, while real-time PCR was used to determine the regulation of selected genes. The results showed that the 14 differentially expressed genes (DEGs) identified were homologous to nine known genes in GenBank. These proteins include fructose biphosphate aldolase A (ALDOA), troponin I (TnI), myosin heavy chain (MYH), myosin light chain 1a(MLC 1a), creatine kinase (CK), ATPase ii subunit 8 (ATP8) and 6 (ATP6), parvalbumin(PV), ribosomal protein L26(RPL26), and L11 (RPL11). Real-time quantitative PCR (qPCR) analysis of TnI, MYH, and PV showed that the highest expression (p  < 0.05) occurred in the 20 ppt salinity treatment group, i.e., 8.79, 3.76, and 1.79 fold more induction than in the control group, respectively. However, the expression of the growth hormone (GH) gene was higher in the 10 ppt salinity treatment than in the control group (p  < 0.05). This study confirmed that salinity exposure induces the expression of several genes in P. nasutus, however, future studies need to unravel the importance of these genes for fish performance.

Abstract Image

盐度诱导的巴沙鱼基因表达(Bleeker, 1863)
本研究旨在评估不同浓度盐暴露对巴沙鱼(Pangasius nasutus)的分子反应。在登嘉奴的已知来源获得了具有相似繁殖史的纳苏特鼠幼鱼(平均体型为35±0.54 cm, 152±1.82 g)。本研究使用的盐度范围(即0,10,15和20ppt)是通过范围测定测试确定的,并且在实验室条件下,将这些选择的盐度范围暴露在3个幼体中,为期2周。随后,收集10个生物重复的肌肉组织进行分子分析。差异显示逆转录酶PCR用于鉴定表达的cDNA片段,实时荧光定量PCR用于确定所选基因的调控作用。结果表明,鉴定的14个差异表达基因(DEGs)与GenBank中已知的9个基因同源。这些蛋白包括果糖二磷酸醛缩酶A (ALDOA)、肌钙蛋白I (TnI)、肌球蛋白重链(MYH)、肌球蛋白轻链1a(MLC 1a)、肌酸激酶(CK)、atp酶ii亚基8 (ATP8)和6(ATP6)、小白蛋白(PV)、核糖体蛋白L26(RPL26)和L11 (RPL11)。实时荧光定量PCR (qPCR)分析TnI、MYH和PV的表达量最高(p <;20 PPT盐处理组诱导率分别为对照组的8.79倍、3.76倍和1.79倍。而生长激素(GH)基因在10 ppt盐处理下的表达量高于对照组(p <;0.05)。本研究证实了盐度暴露诱导了纳苏托鱼中几种基因的表达,然而,未来的研究需要揭示这些基因对鱼类生产性能的重要性。
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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